The surface of fifteen polymethacrylate monolithic stationary phases has been modified by a post-polymerization UV-initiated grafting reaction with bifunctional poly(ethylene glycol)dimethacrylate monomers. An effect of crosslinking monomer length, its concentration in the modification mixture, and a time of the modification reaction have been selected to control the extent of modification by a design of experiments protocol. Hydrodynamic and kinetic properties of prepared columns were characterized by capillary liquid chromatography. Regression analysis of determined data revealed that there is only a minor effect of modification reaction on column permeability, as it is rather controlled by the composition of the polymerization mixture used to prepare generic monolith. On the other hand, the utilization of shorter crosslinking monomer increased the formation of small pores and minimized mass transfer resistance effect. Both column efficiency and mass transfer resistance also improved when a lower concentration of crosslinking monomer in the modification mixture was used. Photografting modification decreased a negative effect of mass transfer resistance related to a crosslink density gradient and allowed fast isocratic separations of dopamine metabolism-related compounds. Developed preparation protocol might be further utilized in the preparation of monolithic stationary phases in microfluidic devices.

十五个聚甲基丙烯酸酯整体固定相的表面已通过聚合后紫外线引发的接枝反应与双官能聚（乙二醇）二甲基丙烯酸酯单体进行改性。已选择交联单体长度、其在改性混合物中的浓度和改性反应时间的影响，以通过实验方案的设计来控制改性程度。用毛细管液相色谱法表征所制备柱的流体动力学和动力学特性。确定数据的回归分析表明，改性反应对柱渗透率的影响很小，因为它更受用于制备通用整体料的聚合混合物的组成控制。另一方面，较短的交联单体的使用增加了小孔的形成并最小化了传质阻力效应。当在改性混合物中使用较低浓度的交联单体时，柱效和传质阻力也有所提高。光接枝修饰降低了与交联密度梯度相关的传质阻力的负面影响，并允许多巴胺代谢相关化合物的快速等度分离。开发的制备方案可能会进一步用于微流体装置中整体固定相的制备。光接枝修饰降低了与交联密度梯度相关的传质阻力的负面影响，并允许多巴胺代谢相关化合物的快速等度分离。开发的制备方案可能会进一步用于微流体装置中整体固定相的制备。光接枝修饰降低了与交联密度梯度相关的传质阻力的负面影响，并允许多巴胺代谢相关化合物的快速等度分离。开发的制备方案可能会进一步用于微流体装置中整体固定相的制备。