Memory and dendritic spines loss, and dynamic dendritic spines changes are age-dependent in the rat,Journal of Chemical Neuroanatomy

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Memory and dendritic spines loss, and dynamic dendritic spines changes are age-dependent in the rat
Journal of Chemical Neuroanatomy ( IF 2.7 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.jchemneu.2020.101858
Leonardo Aguilar-Hernández ₁ , Andrea Judith Vázquez-Hernández ₁ , Diana Frida de-Lima-Mar ₂ , Rubén Antonio Vázquez-Roque ₂ , Hiram Tendilla-Beltrán ₁ , Gonzalo Flores ₂

Affiliation

Brain aging is a widely studied process, but due to its complexity, much of its progress is unknown. There are many studies linking memory loss and reduced interneuronal communication with brain aging. However, only a few studies compare young and old animals. In the present study, in male rats aged 3, 6, and 18 months, we analyzed the locomotor activity and also short and long-term memory using the new object recognition test, in addition to evaluating the dendritic length and the number of dendritic spines in the prefrontal cortex (PFC) and in the CA1, CA3 and DG regions of the hippocampus using Golgi-Cox staining. We also analyzed the types of dendritic spines in the aforementioned regions. 6- and 18-month old animals showed a reduction in locomotor activity, while long-term memory deficit was observed in 18-month old rats. At 18 months old, the dendritic length was reduced in all the studied regions. The dendritic spine number was also reduced in layer 5 of the PFC, and the CA1 and CA3 of the hippocampus. The dynamics of dendritic spines changed with age, with a reduction of the mushroom spines in all the studied regions, with an increase of the stubby spines in all the studied regions except from the CA3 region, that showed a reduction. Our data suggest that age causes changes in behavior, which may be the result of morphological changes at the dendrite level, both in their length and in the dynamics of their spines.

中文翻译：

记忆和树突棘丧失，以及动态树突棘变化在大鼠中与年龄有关

大脑老化是一个被广泛研究的过程，但由于其复杂性，其大部分进展尚不清楚。有许多研究将记忆力减退和神经元间交流减少与大脑衰老联系起来。然而，只有少数研究比较了年轻和年老的动物。在本研究中，除了评估树突长度和树突棘数量外，我们还使用新的物体识别测试分析了 3、6 和 18 个月大的雄性大鼠的运动活动以及短期和长期记忆使用 Golgi-Cox 染色在前额叶皮层 (PFC) 和海马的 CA1、CA3 和 DG 区域。我们还分析了上述区域中树突棘的类型。6 个月和 18 个月大的动物表现出运动活动减少，而在 18 个月大的大鼠中观察到长期记忆缺陷。在 18 个月大时，所有研究区域的树突长度都减少了。PFC 的第 5 层以及海马的 CA1 和 CA3 中的树突棘数量也减少了。树突棘的动态随年龄变化，所有研究区域的蘑菇棘减少，除CA3区域外，所有研究区域的粗刺增加，表明减少。我们的数据表明，年龄会导致行为发生变化，这可能是树突水平形态变化的结果，无论是在它们的长度还是在它们的脊柱动力学方面。随着所有研究区域的蘑菇刺减少，除了 CA3 区域外，所有研究区域的短刺增加，表明减少。我们的数据表明，年龄会导致行为发生变化，这可能是树突水平形态变化的结果，无论是在它们的长度还是在它们的脊柱动力学方面。随着所有研究区域的蘑菇刺减少，除了 CA3 区域外，所有研究区域的短刺增加，表明减少。我们的数据表明，年龄会导致行为发生变化，这可能是树突水平形态变化的结果，无论是在它们的长度还是在它们的脊柱动力学方面。

更新日期：2020-12-01

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