Carbon recovery through polyhydroxybutyrate (PHB) production can create a value-added waste management system. Activated sludge as inoculum enables PHB production using cheap and renewable carbons source, bringing PHB at par to conventional plastics. The PHB accumulating potential of activated sludge needs to be improved to realize the objective. The interaction between the origin of activated sludge, petroleum refinery sludge and brewery sludge, and nitrogen availability was explored to effect culture enrichment, improve PHB accumulation, and polymer characteristics through aerobic dynamic feeding. Consequently, nitrogen excess and limitation enrichment of both sludges produced mix microbial culture with adequate PHB storage of 7.8 ± 0.05%, 14.4 ± 0.04%, 14.4 ± 0.04%, 13.4 ± 0.02% respectively. Batch accumulation revealed higher PHB accumulation of 76.1 ± 0.03% and 71.7 ± 0.05% under nitrogen limitation for PRS and BS enriched under nitrogen excess condition compared to any other combination. The higher decomposition temperature of 285 °C, 293 °C, and a lower melting point of 168 °C, 165 °C with a higher molecular weight of 4.3x10⁵g/mol and semi-crystalline arrangement indicates the potential applications for extracted PHB. PHB production enhanced under nitrogen limited conditions with culture enriched under nitrogen excess condition. However, similar PHB storage, physiochemical property, and overlapping microbial community show an insignificant effect of sludge origin on PHB production.

通过聚羟基丁酸酯（PHB）生产回收碳可以创建增值废物管理系统。活性污泥作为接种物，可以使用廉价的可再生碳源生产PHB，使PHB与传统塑料相当。需要提高活性污泥的PHB积累潜力以实现该目标。探索了活性污泥，炼油厂污泥和啤酒厂污泥的来源与氮素之间的相互作用，以通过有氧动态进料实现培养物富集，改善PHB积累和聚合物特性。因此，两种污泥的氮过量和限制富集产生了混合的微生物培养物，其PHB的充足储量分别为7.8±0.05％，14.4±0.04％，14.4±0.04％，13.4±0.02％。与任何其他组合相比，对于在氮过量条件下富集的PRS和BS，在氮限制下，批次积累显示出较高的PHB积累，分别为76.1±0.03％和71.7±0.05％。较高的分解温度为285°C，293°C，较低的熔点为168°C和165°C，较高的分子量为4.3x10⁵ g / mol和半结晶排列表明提取的PHB的潜在应用。在氮不足的条件下，通过在氮过量的条件下富集的培养物可提高PHB的产量。但是，类似的PHB储存，理化特性和重叠的微生物群落显示，污泥来源对PHB的生产影响不明显。