During acidic pretreatments, both lignin and pseudo-lignin tend to form aggregated droplets on the surface of cellulose and further inhibit its enzymatic digestibility. However, the interaction performance between pseudo-lignin and enzymes is still obscure. In this work, acid pretreated bamboo residues (AP-BR) were extracted with 1, 4-dioxane to remove these droplets, which decreased the lignin content and increased the glucose yield from 16.3 % to 23.0 %. To further investigate these droplets on the enzymatic hydrolysis, the adsorption capability of pseudo-lignin and residual lignin on the enzymes were measured by quartz crystal microgravimetry with dissipation monitoring. The results indicated that pseudo-lignin has similar adsorption capability to enzymes as lignin in the fast adsorption process, but residual lignin in AP-BR (PMWL) and pseudo-lignin generated from glucose (GPL) take longer time in slow adsorption process than the pseudo-lignin generated from xylose (XPL). Besides, lignin showed faster desorption capability than pseudo-lignin. It is also interesting to find that lignin and pseudo-lignin exhibited lower adsorption capability toward enzymes than cellulose. According to the dynamics of enzymatic hydrolysis, when pseudo-lignin and lignin were mixed with cellulose, the maximum amount of products could be reduced and it would take longer time to reaching the maximum enzymatic hydrolysis efficiency than neat cellulose. This work provides an insight into how the pseudo-lignin /lignin droplets affect the enzymatic digestibility of pretreated biomass.

在酸性预处理期间，木质素和假木质素都倾向于在纤维素表面上形成聚集的液滴，并进一步抑制其酶消化性。但是，伪木质素和酶之间的相互作用仍然不清楚。在这项工作中，酸预处理的竹残渣（AP-BR）用1，4-二恶烷萃取以除去这些液滴，从而降低了木质素含量并使葡萄糖产率从16.3％提高到23.0％。为了进一步研究这些液滴在酶促水解中的作用，采用耗散监测的石英晶体微重力法测量了假木质素和残留木质素在酶上的吸附能力。结果表明，在快速吸附过程中，伪木质素的吸附能力与木质素相似，与酶类似。但是，AP-BR（PMWL）中残留的木质素和葡萄糖（GPL）生成的伪木质素在缓慢的吸附过程中比木糖（XPL）生成的伪木质素需要更长的时间。此外，木质素比假木质素具有更快的解吸能力。还有趣的是，木质素和假木质素对纤维素的酶吸附能力比纤维素低。根据酶促水解的动力学，当将伪木质素和木质素与纤维素混合时，可以减少最大量的产物，并且比纯纤维素需要更长的时间才能达到最大的酶促水解效率。这项工作提供了对伪木质素/木质素液滴如何影响预处理的生物质的酶消化率的见解。