Sugarcane straw was pretreated with acetic acid in a microwave-assisted process for further use in fermentative butyric acid production by Clostridium beijerinckii Br21. An experimental design with temperatures between 170 and 220 °C and acetic acid concentrations between 0.3 and 0.9 % (w/v) provided the optimal conditions (220 °C and 0.3 % acetic acid (w/v)) for maximum total sugar recovery (74 %). Three hydrolysates originated from sugarcane straw pretreatment under optimal conditions were used as substrates for fermentation to produce butyric acid. Hydrolysate 1 was the liquid fraction resulting from pretreatment; Hydrolysate 2 was the enzymatic hydrolysate of the pretreated solid fraction, whereas Hydrolysate 3 was the enzymatic hydrolysate obtained from the whole slurry. In all the fermentations in which the hydrolysates were employed, butyric acid was the only product that was generated by C. beijerinckii Br21: 19.5 g/L was the maximum concentration obtained, and the yield was 0.46 g of butyric acid per g of sugar. C. beijerinckii Br21 was also able to consume the acetic acid that was used in the pretreatment step. Therefore, sugarcane straw pretreatment by microwave in the presence of acetic acid is a promising method to obtain highly specific butyric acid bioproduction.

在微波辅助工艺中，用乙酸对甘蔗秸秆进行了预处理，以进一步用于拜氏梭菌的发酵丁酸生产Br21。温度在170至220°C之间且乙酸浓度在0.3至0.9％（w / v）之间的实验设计为最佳的总糖回收率提供了最佳条件（220°C和0.3％的乙酸（w / v））（ 74％）。在最佳条件下将源自甘蔗秸秆预处理的三种水解物用作发酵底物以生产丁酸。水解产物1是预处理产生的液体馏分；水解产物2是预处理的固体级分的酶促水解产物，而水解产物3是从整个浆液获得的酶促水解产物。在所有使用水解产物的发酵中，丁酸是由拜氏梭菌产生的唯一产物Br 21：获得的最大浓度为19.5g / L，每克糖的产量为0.46克丁酸。拜氏梭菌Br21还能够消耗在预处理步骤中使用的乙酸。因此，在乙酸存在下通过微波对甘蔗秸秆进行预处理是获得高特异性丁酸生物生产的有前途的方法。