Abstract For value-added utilization for wood constitute of galactose, which is a kind of hard fermentable lignocellulosic C6 sugar compared with glucose and mannose, a simple and efficient bioprocess for directed regulation of galactonic acid bio-preparation with satisfactory yield and efficiency was described by whole-cell catalysis with native bacterial strain of Gluconobacter oxydans. During the whole-cell catalysis, by employing Ca(OH)2 as neutralizer, 97.7 g of high-quality calcium galactonate was prepared efficiently within 24 h with the productivity of 4.1 g/h, and the serious damage to the product quality caused by the by-product of 2-keto-galactonic acid was completely avoided. As results, based on the consumption of sugar, we successfully achieved 96.9% yield of high purity galactose acid (salts). Moreover, the crystal structure of calcium galactonate was especially prepared and detected by mass spectrometry and 13C NMR in view of relevant studies, as well as standard chemical with high purity for the determination and analysis in chemical and biological fluids. Finally, the biotechnology provides a promising approach for utilization of galactose and promotes application of high purity galactonic acid (salts) in chemical and biological fields.

中文翻译：

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Ca2+ 对全细胞催化高质量半乳糖酸生物制备和表征的定向调控

摘要 半乳糖是一种与葡萄糖和甘露糖相比的硬质可发酵木质纤维素 C6 糖，对于木材构成的增值利用，描述了一种简单有效的生物过程，用于直接调节半乳糖酸生物制备，并具有令人满意的收率和效率。用氧化葡糖杆菌的天然细菌菌株进行全细胞催化。在全细胞催化过程中，以Ca(OH)2为中和剂，在24h内高效制备了97.7g优质半乳糖酸钙，产率为4.1g/h，严重损害了产品质量。完全避免了 2-酮-半乳糖酸的副产物。结果，基于糖的消耗量，我们成功地实现了 96.9% 的高纯度半乳糖酸（盐）收率。而且，半乳糖酸钙的晶体结构是针对相关研究，特别制备并通过质谱和13C NMR检测的，以及用于化学和生物流体中测定和分析的高纯度标准化学品。最后，生物技术为半乳糖的利用提供了一种有前景的途径，并促进了高纯度半乳糖酸（盐）在化学和生物领域的应用。