A series of unsaturated (16-electron) five-coordinated ruthenium (II) complexes of the type [(η⁶-arene)Ru(N^N)]⁺PF₆⁻, where N^N is aminoimine and η⁶-arene is benzene (bz) (Ru1), 3-phenylpropan-1-ol (bz-PA) (Ru2), or 4-phenylbutan-1-ol (bz-BA) (Ru3), respectively, have been serendipitously synthesized via solvent (methanol)-involved rearrangement. The identity and purity of these new complexes were determined by NMR spectroscopy, elemental analysis, mass spectrometry, and X-ray crystallography techniques. Their fluorescence property and electrochemical behavior were also investigated. These complexes showed sufficient stability and exhibited higher anticancer activity toward A549 human lung cancer cells, human cervical carcinoma cells (HeLa) and hepatocellular carcinoma cell (HepG2) than cisplatin. The investigation of structure-activity relationship displayed that Ru1 with the hydrophobic groups exhibited enhanced anticancer activity in comparison to Ru2 and Ru3 containing hydroxyl groups. Notably, complex Ru1 entered A549 human lung cancer cells through a non-energy-dependent pathway and mainly accumulated in lysosomes. Moreover, Ru1 induced a dose-dependent apoptosis and sub-G1 fraction perturbation.

一系列不饱和的（16-电子）的五配位的钌（II）的类型的配合物[（η ⁶ -arene）的Ru（N ^ N）] ⁺ PF ₆ ^- ，其中N 1，N是aminoimine和η ⁶ -arene是苯（bz）（Ru1），3-苯基丙-1-醇（bz-PA）（Ru2）或4-苯基丁-1-醇（bz-BA）（Ru3）分别是通过溶剂（甲醇）相关的重排意外合成的。这些新配合物的身份和纯度通过NMR光谱，元素分析，质谱和X射线晶体学技术确定。还研究了它们的荧光性质和电化学行为。这些复合物显示出足够的稳定性，并且比顺铂对A549人肺癌细胞，人宫颈癌细胞（HeLa）和肝细胞癌细胞（HepG2）具有更高的抗癌活性。结构-活性关系的研究表明，与Ru2和Ru3相比，具有疏水基团的Ru1具有增强的抗癌活性。含有羟基。值得注意的是，复杂的Ru1通过非能量依赖性途径进入A549人肺癌细胞，并且主要在溶酶体中积累。此外，Ru1诱导剂量依赖性凋亡和亚G1分数扰动。