Cranial and craniospinal irradiation are the oldest central nervous system prophylaxis treatments considered for pediatric patients with acute lymphoblastic leukemia (ALL). However, survivors of childhood ALL that received cranial radiotherapy are at increased risk for deficits in neurocognitive skills. The continuous and dynamic response of normal tissue after irradiation has been identified as one of the causative factors for cognitive changes after cranial radiation therapy. The aim of our study was to investigate the radiation effects on social behavior and neuronal morphology in the hippocampus of adult mice. Twenty-oneday-old male C57BL/6 mice were irradiated with the small-animal radiation research platform (SARRP). Animals were given a single 10-Gy dose of radiation of X-ray cranial radiation. One month following irradiation, animals underwent behavioral testing in the Three-Chamber Sociability paradigm. Radiation affected social discrimination during the third stage eliciting an inability to discriminate between the familiar and stranger mouse, while sham successfully spent more time exploring the novel stranger. Proteomic analysis revealed dysregulation of metabolic and signaling pathways associated with neurocognitive dysfunction such as mitochondrial dysfunction, Rac 1 signaling, and synaptogenesis signaling. We observed significant decreases in mushroom spine density in the Cornu Ammonis 2 of the hippocampus, which is associated with sociability processing.

颅骨和颅脊髓照射是考虑用于急性淋巴细胞白血病 (ALL) 儿科患者的最古老的中枢神经系统预防治疗。然而，接受颅脑放疗的儿童 ALL 幸存者出现神经认知技能缺陷的风险增加。正常组织在照射后的持续动态反应已被确定为颅脑放射治疗后认知改变的诱因之一。我们研究的目的是调查辐射对成年小鼠海马体的社会行为和神经元形态的影响。21 天大的雄性 C57BL/6 小鼠用小动物辐射研究平台 (SARRP) 进行照射。给予动物单次 10-Gy 剂量的 X 射线颅骨辐射。照射后一个月，动物在三室社交范式中进行了行为测试。辐射在第三阶段影响社会歧视，导致无法区分熟悉的和陌生的老鼠，而假手术成功地花了更多时间探索新奇的陌生人。蛋白质组学分析揭示了与神经认知功能障碍相关的代谢和信号通路失调，例如线粒体功能障碍、Rac 1 信号传导和突触发生信号传导。我们观察到海马的 Cornu Ammonis 2 中蘑菇棘密度显着降低，这与社交处理有关。而 sham 成功地花了更多的时间来探索这个小说陌生人。蛋白质组学分析揭示了与神经认知功能障碍相关的代谢和信号通路失调，例如线粒体功能障碍、Rac 1 信号传导和突触发生信号传导。我们观察到海马的 Cornu Ammonis 2 中蘑菇棘密度显着降低，这与社交处理有关。而 sham 成功地花了更多的时间来探索这个小说陌生人。蛋白质组学分析揭示了与神经认知功能障碍相关的代谢和信号通路失调，例如线粒体功能障碍、Rac 1 信号传导和突触发生信号传导。我们观察到海马的 Cornu Ammonis 2 中蘑菇棘密度显着降低，这与社交处理有关。