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Comparative analysis of the constituents of two cultivars of Dioscorea dumetorum (Kunth) Pax. and their molecular barcoding
Biochemical Systematics and Ecology ( IF 1.6 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.bse.2020.104140
Adedapo Adedayo Adeniran , Mubo Adeola Sonibare , Shashi Kumar

Abstract The edible and wild cultivars of Dioscorea dumetorum (Kunth) Pax. (Family: Dioscoreaceae) are commonly used as close substitutes in herbal markets due to their edibility and medicinal importance. Comparative study of the cultivars was done using chemical analysis of their methanol tuber extracts and by utilizing the DNA barcode regions, ribulose-1, 5-bisphosphate carboxylase subunits (rbcL) and the non-coding intergenic spacer gene (trnH-psbA) for nucleotide sequences comparison of cultivars. Of the 54 compounds identified from the methanol extract of the edible and wild cultivars using gas chromatography-mass spectrometry (GC-MS), 12 are similar, while 42 are remarkably different. In particular n-hexadecanoic (palmitic) acid had a higher percentage area in the edible cultivar (31.16%) compared to the wild cultivar (0.26%). The cultivars were successfully amplified using the universal primers gene rbcL and trnH-psbA. Their nucleotide sequences showed a slight variation when aligned with CodonCode Aligner V.9.0.1. They were identified by the Basic Local Alignment Search Tool (BLAST) through comparison with Genbank data, which shows close similarities with submitted data. The use of molecular barcoding in identifying the cultivars of Dioscorea dumetorum as well as the chemical composition of their tubers may form an important marker in the compilation of future herbal pharmacopoeia for its proper identification.

中文翻译:

Dioscorea dumetorum (Kunth) Pax 两个品种成分的比较分析。和他们的分子条形码

摘要 Dioscorea dumetorum (Kunth) Pax 的食用和野生品种。(科:薯蓣科)由于其可食用性和药用价值,通常被用作草药市场的紧密替代品。通过对其甲醇块茎提取物进行化学分析,并利用 DNA 条形码区域、核酮糖-1、5-二磷酸羧化酶亚基 (rbcL) 和核苷酸的非编码基因间间隔基因 (trnH-psbA),对栽培品种进行了比较研究。品种序列比较。使用气相色谱-质谱法 (GC-MS) 从可食用和野生栽培品种的甲醇提取物中鉴定出的 54 种化合物中,有 12 种相似,而 42 种有显着差异。特别是,与野生品种(0.26%)相比,正十六烷酸(棕榈酸)在可食用品种(31.16%)中的面积百分比更高。使用通用引物基因rbcL和trnH-psbA成功扩增了栽培品种。当与 CodonCode Aligner V.9.0.1 比对时,它们的核苷酸序列显示出轻微的变化。它们是由基本局部比对搜索工具 (BLAST) 通过与 Genbank 数据进行比较而确定的,该数据显示出与提交的数据非常相似。分子条形码技术在鉴别薯蓣品种及其块茎的化学成分方面的应用,可能成为未来草本药典编纂以进行正确鉴别的重要标志。它们是由基本局部比对搜索工具 (BLAST) 通过与 Genbank 数据进行比较而确定的,该数据显示出与提交的数据非常相似。分子条形码技术在鉴别薯蓣品种及其块茎的化学成分方面的应用,可能成为未来草本药典编纂以进行正确鉴别的重要标志。它们是由基本局部比对搜索工具 (BLAST) 通过与 Genbank 数据进行比较而确定的,该数据显示出与提交的数据非常相似。分子条形码技术在鉴别薯蓣品种及其块茎的化学成分方面的应用,可能成为未来草本药典编纂以进行正确鉴别的重要标志。
更新日期:2020-12-01
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