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Production and Assessment of Potato Material Resistant to the Broadleaf Herbicide Bentazone
Potato Research ( IF 2.9 ) Pub Date : 2020-09-17 , DOI: 10.1007/s11540-020-09476-8
Bo Tao , Shiqi Sun , Lili Zhang , Jing Guo , Baihui Shao

In potato cultivation, the problem of controlling broadleaf weeds could be solved with the production of plants resistant to the broadleaf herbicide bentazone. Plant tissue culture technology and herbicide screening technology were used to select bentazone-resistant potato materials and to analyse the physiological and biochemical characteristics of their resistance. Potato microtubers were used as explants. Results showed that the optimum medium for screening the resistance was the Murashige and Skoog medium + 1 mg/L indoleacetic acid + 4 mg/L zeatin + 60 mg/L bentazone (pH 5.8). Forty-one regenerated buds were obtained by screening culture. After treatment with 48% bentazone aqueous solution (700 mg/L and 1296 g a.i./ha), one resistant product was obtained. Exposure to bentazone (1296 g a.i./ha) reduced the physiological indices of both the resistant and control potato plants. However, compared with control, the photosynthetic indices and acetyl-CoA carboxylase activity of resistant selection only decreased slightly and increased significantly from the lowest level, and the content of malondialdehyde, an indicator of plant stress, was also less affected.



中文翻译:

马铃薯抗阔叶除草剂倍他酮的生产与评价

在马铃薯栽培中,防治阔叶杂草的问题可以通过生产对阔叶除草剂苯达松有抗性的植物来解决。利用植物组织培养技术和除草剂筛选技术,选择耐苯达松的马铃薯材料,并对其抗性进行生理生化分析。马铃薯微型块茎用作外植体。结果表明,筛选抗药性的最佳培养基是Murashige和Skoog培养基+ 1 mg / L吲哚乙酸+ 4 mg / L玉米素和60 mg / L苯达松(pH 5.8)。通过筛选培养获得41个再生芽。用48%的bendazone水溶液(700 mg / L和1296 g ai / ha)处理后,获得了一种抗药性产品。暴露于bentazone(1296 g ai / ha)降低了抗性和对照马铃薯植株的生理指标。然而,与对照相比,抗性选择的光合作用指数和乙酰辅酶A羧化酶活性仅从最低水平略有下降,并显着增加,而丙二醛含量(植物胁迫指标)的影响也较小。

更新日期:2020-09-18
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