In this study, the chemical profile of Ling-Gui-Zhu-Gan Decoction (LGZGD) which was prepared by the method recorded in Synopsis of Golden Chamber was analyzed by LC–QTOF MS. A total of 89 compounds were identified, including flavonoids, pentacyclic triterpenes, glycosides, organic acids and others. Among them, nine major active components with high response value in chromatogram were selected for quantification, including liquiritin apioside, liquiritin, isoliquiritin apioside, isoliquiritin, coumarin, liquiritigenin, cinnamic acid, cinnamaldehyde and glycyrrhizic acid. A quantitative analysis of multi-components by single marker (QAMS) was applied to simultaneous determination of nine major components in LGZGD. Using liquiritin as the internal reference, the relative correction factors (RCFs) were calculated to determine the contents of other eight compounds. A series of methodological study and robustness study were carried out, which proved that the RCFs between liquiritin and others were stable. In addition, compared with the results calculated by external standard method (EMS), there were no remarkable differences between these two methods with absolute relative errors less than 3.12%. The developed QAMS method was successfully applied to preparation process analysis. In conclusion, this QAMS method was convenient, accurate and feasible enough for evaluating the quality of LGZGD.

中文翻译：

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采用 LC-QTOF MS 分析灵桂竹甘汤的化学特征并使用 QAMS 方法同时测定九种主要成分

在本研究中，通过 LC-QTOF MS 分析了通过《金室概要》中记录的方法制备的灵桂竹甘汤 (LGZGD) 的化学特征。共鉴定出89种化合物，包括黄酮类、五环三萜、苷类、有机酸等。其中，选取色谱中响应值高的9种主要活性成分进行定量，包括甘草苷、甘草苷、异甘草苷、异甘草苷、香豆素、甘草素、肉桂酸、肉桂醛和甘草酸。单标记多组分定量分析 (QAMS) 用于同时测定 LGZGD 中的九种主要成分。以甘草苷为内参，计算相对校正因子 (RCF) 以确定其他八种化合物的含量。进行了一系列的方法学研究和稳健性研究，证明甘草苷与其他物质的RCFs是稳定的。此外，与外标法（EMS）计算的结果相比，这两种方法的计算结果没有显着差异，绝对相对误差小于3.12%。开发的 QAMS 方法已成功应用于制备过程分析。综上所述，该 QAMS 方法对于 LGZGD 的质量评价足够方便、准确和可行。与外标法（EMS）计算的结果相比，这两种方法的计算结果没有显着差异，绝对相对误差小于3.12%。开发的 QAMS 方法已成功应用于制备过程分析。综上所述，该 QAMS 方法对于 LGZGD 的质量评价足够方便、准确和可行。与外标法（EMS）计算的结果相比，这两种方法的计算结果没有显着差异，绝对相对误差小于3.12%。开发的 QAMS 方法已成功应用于制备过程分析。综上所述，该 QAMS 方法对于 LGZGD 的质量评价足够方便、准确和可行。