To investigate the alleviating effects of low intensity pulsed ultrasound (LIPUS) on myelosuppression of Sprague-Dawley rats with breast cancer induced by cyclophosphamide (CTX). Breast cancer on rats was triggered by intragastric gavage with 7, 12-dimethylbenz[a]anthracene (150 mg/kg). Then the rats with breast cancer were randomly allocated to the LIPUS group (n = 50) and the control group (n = 50). The LIPUS group was injected intraperitoneally with CTX (50 mg/kg) for 4 consecutive days and underwent LIPUS treatment at femoral metaphysis 20 minutes per day from the first day of injection for 7 consecutive days. The control group was injected with CTX (50 mg/kg) and treated with LIPUS without energy output. Blood, enzyme-linked immunosorbent assay, real-time quantitative polymerase chain reaction, hematoxylin & eosin staining and scanning electron microscope were applied to detect the changes. The results indicated LIPUS significantly promoted the proliferation of bone marrow nucleated cells, white blood cells, IgA, IgG, and IgM in the peripheral blood (p < 0.05) without the damage to liver and kidney function simultaneously. The mechanisms may result from the LIPUS alleviation effect on bone marrow hematopoietic function through regulating cytokines such as LIPUS can increase the expression of granulocyte colony-stimulating factor, stem cell factor, transforming growth factor -β, and intercellular cell adhesion molecule-1, meanwhile LIPUS will decrease the expression of interleukin-6, tumor necrosis factor -α, and vascular cell adhesion molecule-1. LIPUS has potential to be a new adjuvant therapy method in clinic for ameliorating chemotherapy-induced myelosuppression.

中文翻译：

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通过低强度脉冲超声改善乳腺癌大鼠治疗过程中环磷酰胺诱导的骨髓抑制。

目的研究低强度脉冲超声（LIPUS）对环磷酰胺（CTX）诱导的乳腺癌Sprague-Dawley大鼠骨髓抑制的缓解作用。用7、12-二甲基苯并[a]蒽（150 mg / kg）灌胃对大鼠造成乳腺癌。然后将乳腺癌大鼠随机分为LIPUS组（n = 50）和对照组（n = 50）。LIPUS组连续4天腹膜内注射CTX（50 mg / kg），并从注射第一天起每天每天20分钟在股骨干physi端进行LIPUS治疗，连续7天。对照组注射CTX（50 mg / kg），并用LIPUS治疗，无能量输出。血液，酶联免疫吸附测定，实时定量聚合酶链反应，苏木精和 曙红染色和扫描电镜观察其变化。结果表明，LIPUS可显着促进外周血中的骨髓有核细胞，白细胞，IgA，IgG和IgM的增殖（p <0.05），而不会同时损害肝和肾功能。LIPUS通过调节细胞因子对骨髓造血功能的缓解作用可能是由这些机制引起的，例如LIPUS可以增加粒细胞集落刺激因子，干细胞因子，转化生长因子-β和细胞间细胞粘附分子-1的表达。 LIPUS会降低白介素6，肿瘤坏死因子-α和血管细胞粘附分子1的表达。