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Different binding mechanisms of Staphylococcus aureus to hydrophobic and hydrophilic surfaces.
Nanoscale ( IF 5.8 ) Pub Date : 2020-09-16 , DOI: 10.1039/d0nr03134h
Erik Maikranz 1 , Christian Spengler , Nicolas Thewes , Alexander Thewes , Friederike Nolle , Philipp Jung , Markus Bischoff , Ludger Santen , Karin Jacobs
Affiliation  

Bacterial adhesion to surfaces is a crucial step in initial biofilm formation. In a combined experimental and computational approach, we studied the adhesion of the pathogenic bacterium Staphylococcus aureus to hydrophilic and hydrophobic surfaces. We used atomic force microscopy-based single-cell force spectroscopy and Monte Carlo simulations to investigate the similarities and differences of adhesion to hydrophilic and hydrophobic surfaces. Our results reveal that binding to both types of surfaces is mediated by thermally fluctuating cell wall macromolecules that behave differently on each type of substrate: on hydrophobic surfaces, many macromolecules are involved in adhesion, yet only weakly tethered, leading to high variance between individual bacteria, but low variance between repetitions with the same bacterium. On hydrophilic surfaces, however, only few macromolecules tether strongly to the surface. Since during every repetition with the same bacterium different macromolecules bind, we observe a comparable variance between repetitions and different bacteria. We expect these findings to be of importance for the understanding of the adhesion behaviour of many bacterial species as well as other microorganisms and even nanoparticles with soft, macromolecular coatings, used e.g. for biological diagnostics.

中文翻译:

金黄色葡萄球菌与疏水和亲水表面的不同结合机制。

细菌对表面的粘附是初始生物膜形成的关键步骤。在组合的实验和计算方法中,我们研究了致病菌金黄色葡萄球菌的粘附亲水和疏水表面。我们使用基于原子力显微镜的单细胞力光谱法和蒙特卡洛模拟法研究了对亲水性和疏水性表面粘附的异同。我们的结果表明,两种类型表面的结合都是通过热波动的细胞壁大分子介导的,这些分子在每种类型的底物上的行为各不相同:在疏水性表面上,许多大分子参与粘附,但只有弱束缚,导致各个细菌之间的差异很大,但使用同一细菌的重复之间的差异很小。然而,在亲水表面上,只有少数大分子牢固地束缚在表面上。由于在同一细菌的每次重复过程中,不同的大分子都会结合在一起,我们观察到重复与不同细菌之间的可比方差。我们希望这些发现对于理解许多细菌物种以及其他微生物甚至具有柔软的大分子涂层的纳米颗粒的粘附行为具有重要意义。例如用于生物学诊断。
更新日期:2020-10-02
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