Pathogenic bacteria that recently infect humans have been undergone an evolution. The evolution of treatment measures is not in accordance with the dose. Several cases in hospitals have found multi-type bacterial drug resistance. These cases affected much death of the patient. Therefore, this study explores the seaweed from the Karimunjawa Islands, Jepara as the potential sources of new antibacterial active compounds. The study is conducted in the laboratory and used the experimental design of sampling, extraction, resistance test, antibacterial extract test, and analysis of extract active compounds. There are three types of seaweed sampled: S. crassifolium, Padina australis and Dictyota dichotoma. Each extraction is carried out with diethyl ether, methanol, ethanol and chloroform solvents. This study used pathogenic bacterial isolates: Pseudomonas aeruginosa, Staphylococcus epidermidis, Staphylococcus aureus and Escherichia coli which are tested with 13 antibiotics. The resistance test showed the value of multiple antibiotic resistance index (mar) of 0.84; 0.76; 0.48 and 0.34. Antibiotics with a high percentage resistance value (100%) are from groups of lipopeptides (colistine sulfate) and (3-lactams groups (ampicillin). The seaweeds extract tested for antibacterial activity at different concentrations. The results showed that different types and concentrations of extracts had different antibacterial activity values (p <0.05). The extract with high antibacterial activity is an extract from seaweed S crassifolium with methanol solvent. The extract contained phenol 12 mg/g, flavonoid 1.6%, tannin 0.63%, fucoxanthin 0.59 mg/g, carotenoid 165.9 g/g, chlorophyll-a 0.92 mg/g and chlorophyll-b 0.28 mg/g.

最近感染人类的​​病原菌已经经历了进化。治疗措施的演变与剂量不符。几例医院发现多型细菌耐药。这些病例影响了患者的大量死亡。因此，本研究探讨了来自 Jepara 的 Karimunjawa 群岛的海藻作为新型抗菌活性化合物的潜在来源。该研究在实验室进行，采用了取样、提取、抗性测试、抗菌提取物测试和提取物活性化合物分析的实验设计。采样了三种海藻：S. crassifolium、Padina australis和Dictyota dichotoma。每次萃取均用乙醚、甲醇、乙醇和氯仿溶剂进行。本研究使用的致病菌分离物：铜绿假单胞菌、表皮葡萄球菌、金黄色葡萄球菌和大肠杆菌用 13 种抗生素进行了测试。耐药性试验显示，多重抗生素耐药指数（mar）为0.84；0.76; 0.48 和 0.34。具有高百分比耐药值（100%）的抗生素来自脂肽组（硫酸粘菌素）和（3-内酰胺组（氨苄青霉素）。海藻提取物在不同浓度下进行了抗菌活性测试。结果表明，不同类型和浓度的提取物具有不同的抗菌活性值（p <0.05）。抗菌活性高的提取物是海藻用甲醇溶剂提取的提取物。提取物含酚12 mg/g、黄酮1.6%、单宁0.63%、岩藻黄质0.59 mg/ g，类胡萝卜素 165.9 g/g，叶绿素-a 0.92 mg/g 和叶绿素-b 0.28 mg/g。