Entada phaseoloides (L.) Merr. is an important traditional medicinal plant. The stem of Entada phaseoloides is popularly used as traditional medicine because of its significance in dispelling wind and dampness and remarkable anti-inflammatory activities. Triterpenoid saponins are the major bioactive compounds of Entada phaseoloides. However, genomic or transcriptomic technologies have not been used to study the triterpenoid saponin biosynthetic pathway in this plant. We performed comparative transcriptome analysis of the root, stem, and leaf tissues of Entada phaseoloides with three independent biological replicates and obtained a total of 53.26 Gb clean data and 116,910 unigenes, with an average N50 length of 1218 bp. Putative functions could be annotated to 42,191 unigenes (36.1%) based on BLASTx searches against the Non-redundant, Uniprot, KEGG, Pfam, GO, KEGG and COG databases. Most of the unigenes related to triterpenoid saponin backbone biosynthesis were specifically upregulated in the stem. A total of 26 cytochrome P450 and 17 uridine diphosphate glycosyltransferase candidate genes related to triterpenoid saponin biosynthesis were identified. The differential expressions of selected genes were further verified by qPT-PCR. The dataset reported here will facilitate the research about the functional genomics of triterpenoid saponin biosynthesis and genetic engineering of Entada phaseoloides.

中文翻译：

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Entada菜豆根，茎和叶组织的转录组比较分析揭示了涉及三萜皂苷生物合成的潜在基因。

Entada菜豆（L.）是重要的传统药用植物。Entada phaseoloides的茎被广泛用作传统药物，因为它具有消除风湿的作用和显着的消炎作用。三萜类皂苷是Entada菜豆的主要生物活性化合物。然而，基因组或转录组技术尚未用于研究该植物中三萜皂苷的生物合成途径。我们用三个独立的生物学重复进行了对Entada菜豆的根，茎和叶组织的转录组分析，并获得了总共53.26 Gb干净数据和116,910个单基因，平均N50长度为1218 bp。根据针对非冗余，Uniprot，KEGG，BUG和BLASTx的搜索，可以将推定功能注释为42,191个单基因（占36.1％）Pfam，GO，KEGG和COG数据库。与三萜类皂苷骨架生物合成有关的大多数单基因在茎中被特异性上调。共鉴定了与三萜皂苷生物合成有关的26种细胞色素P450和17种尿苷二磷酸糖基转移酶候选基因。通过qPT-PCR进一步验证了所选基因的差异表达。此处报告的数据集将有助于研究三萜类皂苷的生物合成功能基因组学和菜豆虫的基因工程。共鉴定了与三萜皂苷生物合成相关的26种细胞色素P450和17种尿苷二磷酸糖基转移酶候选基因。通过qPT-PCR进一步验证了所选基因的差异表达。此处报道的数据集将有助于研究三萜类皂苷的生物合成功能基因组学和菜豆虫的基因工程。共鉴定了与三萜皂苷生物合成相关的26种细胞色素P450和17种尿苷二磷酸糖基转移酶候选基因。通过qPT-PCR进一步证实了所选基因的差异表达。此处报道的数据集将有助于研究三萜皂苷的生物合成功能基因组学和菜豆虫的基因工程。