Introduction: Silver nanoparticles (AgNPs) have been extensively used in wound healing applications owing to their valuable physicochemical and biological properties. The main objective of this study was to evaluate the combined effects of green-synthesized silver nanoparticles (G-AgNPs) and photobiomodulation (PBM; laser irradiation at 830 nm with 5 J/cm²) in normal wounded and diabetic wounded fibroblast cells (WS1).
Methods: The combined effect of G-AgNPs and PBM was studied by various in vitro wound healing studies including cell morphology, cell migration rate and percentage wound closure, cell viability, cell proliferation, and filamentous (F)-actin and nuclear morphology staining.
Results: Cell viability results revealed good cellular compatibility of G-AgNPs to WS1 cells. The combined therapy of G-AgNPs and PBM demonstrated promising results to achieve progressive migration and wound closure in both normal wounded and diabetic wounded cell models. G-AgNPs alone and in combination with PBM had no negative effect on cell viability and proliferation, and there was an increase in cell migration.
Conclusion: Overall, these findings demonstrate that the combined treatment of G-AgNPs and PBM does not display any adverse effects on wound healing processes in both normal wounded and diabetic wounded cell models.

Keywords: green synthesis, silver nanoparticles, photobiomodulation, laser, wound healing, cell migration


中文翻译：

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芦荟对绿色合成银纳米粒子的选择性激光效率及其在正常受伤和糖尿病受伤成纤维细胞中的伤口愈合活性：体外研究

简介：银纳米粒子 (AgNPs) 因其宝贵的物理化学和生物学特性而被广泛用于伤口愈合应用。本研究的主要目的是评估绿色合成银纳米粒子 (G-AgNPs) 和光生物调节 (PBM; 激光照射 830 nm，5 J/cm ² ) 在正常受伤和糖尿病受伤的成纤维细胞 (WS1 ）。
方法：通过各种体外伤口愈合研究，包括细胞形态、细胞迁移率和伤口闭合百分比、细胞活力、细胞增殖以及丝状 (F)-肌动蛋白和核形态染色，研究 G-AgNPs 和 PBM 的联合作用。
结果：细胞活力结果显示 G-AgNPs 与 WS1 细胞具有良好的细胞相容性。G-AgNPs 和 PBM 的联合治疗证明了在正常受伤和糖尿病受伤细胞模型中实现渐进性迁移和伤口闭合的有希望的结果。G-AgNPs 单独和与 PBM 组合对细胞活力和增殖没有负面影响，并且细胞迁移增加。
结论：总体而言，这些研究结果表明，G-AgNPs 和 PBM 的联合治疗对正常受伤和糖尿病受伤细胞模型的伤口愈合过程没有任何不利影响。

关键词：绿色合成，纳米银，光生物调节，激光，伤口愈合，细胞迁移