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Single Cell RNA Expression Analysis Using Flow Cytometry Based on Specific Probe Ligation and Rolling Circle Amplification.
ACS Sensors ( IF 8.2 ) Pub Date : 2020-09-16 , DOI: 10.1021/acssensors.0c01569
Yansong Zhao 1 , Chen Lin 1 , Pengcheng Wu 1 , Xiaoyuan Chen 1 , Yuancun Zhao 2 , Yupeng Li 2 , Lu Chen 2 , Mats Nilsson 3 , Rongqin Ke 1
Affiliation  

Conventional flow cytometry has been widely used for high-throughput single-cell gene expression analysis using specific antibody staining. However, this is limited by the availability of high-quality antibodies. We developed a novel flow cytometry RNA detection technique termed RCA-Flow for single-cell RNA expression analysis. We showed that it is able to analyze not only mRNAs but also microRNAs and circular RNAs that are otherwise difficult to analyze by other flow cytometry techniques. The versatility for high-throughput analysis of different types of RNA molecules makes our method possess great potential for both biomedical and clinical applications.

中文翻译:

使用基于特定探针连接和滚环扩增的流式细胞术进行单细胞RNA表达分析。

常规流式细胞术已广泛用于使用特异性抗体染色的高通量单细胞基因表达分析。但是,这受到高质量抗体可用性的限制。我们开发了一种新颖的流式细胞术RNA检测技术,称为RCA-Flow,用于单细胞RNA表达分析。我们证明了它不仅能够分析mRNA,而且还能分析其他流式细胞仪技术难以分析的microRNA和环状RNA。高通量分析不同类型的RNA分子的多功能性使我们的方法在生物医学和临床应用中都具有巨大的潜力。
更新日期:2020-10-25
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