Autofluorescence is a long-standing problem that has hindered the analysis of images of tissues acquired by fluorescence microscopy. Current approaches to mitigate autofluorescence in tissue are lab-based and involve either chemical treatment of sections or specialized instrumentation and software to ‘unmix’ autofluorescent signals. Importantly, these approaches are pre-emptive and there are currently no methods to deal with autofluorescence in acquired fluorescence microscopy images.

中文翻译：

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AFid：一种自动识别和从显微镜图像中排除自发荧光物体的工具。

自体荧光是一个长期存在的问题，它阻碍了对通过荧光显微镜获得的组织图像进行分析。当前减轻组织中自体荧光的方法是基于实验室的，涉及对切片进行化学处理或专门的仪器和软件来“分解”自体荧光信号。重要的是，这些方法是先发制人的，并且目前还没有方法在采集的荧光显微镜图像中处理自发荧光。