Profiles of combat injuries worldwide have shown that penetrating trauma is one of the most common injuries sustained during battle. This is usually accompanied by severe bleeding or hemorrhage. If the soldier does not bleed to death, he may eventually succumb to complications arising from trauma hemorrhagic shock (THS). THS occurs when there is a deficiency of oxygen reaching the organs due to excessive blood loss. It can trigger massive metabolic derangements and an overwhelming inflammatory response, which can subsequently lead to the failure of organs and possibly death. A better understanding of the acute metabolic changes occurring after THS can help in the development of interventional strategies, as well as lead to the identification of potential biomarkers for rapid diagnosis of hemorrhagic shock and organ failure. In this preliminary study, a metabolomic approach using the complementary platforms of nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography coupled with mass spectrometry (LC-MS) was used to determine the metabolic changes occurring in a porcine model of combat trauma injury comprising of penetrating trauma to a limb with hemorrhagic shock. Several metabolites associated with the acute-phase reaction, inflammation, energy depletion, oxidative stress, and possible renal dysfunction were identified to be significantly changed after a thirty-minute shock period.

中文翻译：

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使用NMR和多模式LC-MS代谢组学对猪战斗创伤模型进行代谢分析的初步研究。

全世界的战斗伤害情况表明，穿透伤是战斗中最常见的伤害之一。这通常伴有严重的出血或出血。如果士兵没有流血致死，他最终可能会屈服于创伤性出血性休克（THS）引起的并发症。当由于过度失血导致缺氧到达器官时，就会发生THS。它可能引发大量的代谢紊乱和压倒性的炎症反应，继而可能导致器官衰竭甚至死亡。更好地了解THS后发生的急性代谢变化可有助于制定介入策略，并有助于鉴定潜在的生物标志物，以快速诊断失血性休克和器官衰竭。在这项初步研究中，采用了代谢组学方法，利用核磁共振波谱和液相色谱法与质谱联用的互补平台，结合质谱分析法（LC-MS），确定了由以下因素组成的战斗创伤猪模型中发生的代谢变化：出血性休克对四肢的穿透性创伤。与急性期反应，炎症，能量耗竭，氧化应激和可能的肾功能不全相关的几种代谢物在30分钟的休克期后被确定为发生了显着变化。一种代谢组学方法，利用核磁共振（NMR）光谱和液相色谱法与质谱（LC-MS）的互补平台，确定了在战伤模型的猪模型中发生的代谢变化，该模型包括穿透四肢的创伤伴有失血性休克。与急性期反应，炎症，能量耗竭，氧化应激和可能的肾功能不全相关的几种代谢物在30分钟的休克期后被确定为发生了显着变化。一种代谢组学方法，利用核磁共振（NMR）光谱和液相色谱与质谱（LC-MS）的互补平台，确定了在包括创伤穿透性肢体的猪抗击性创伤模型中发生的代谢变化伴有失血性休克。与急性期反应，炎症，能量耗竭，氧化应激和可能的肾功能不全相关的几种代谢物在30分钟的休克期后被确定为发生了显着变化。