Modulating the Stress Response of E. coli at GaN Interfaces Using Surface Charge, Surface Chemistry, and Genetic Mutations,ACS Applied Bio Materials

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Modulating the Stress Response of E. coli at GaN Interfaces Using Surface Charge, Surface Chemistry, and Genetic Mutations
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-09-15 , DOI: 10.1021/acsabm.0c01007
Sara Gleco ₁ , Pramod Reddy ₂ , Ronny Kirste ₂ , Ramón Collazo ₁ , Dennis LaJeunesse ₃ , Albena Ivanisevic ₁

Affiliation

The surface properties of inorganic materials can be used to modulate the response of microorganisms at the interface. We used the persistent photoconductivity properties of chemically treated gallium nitride substrates to evaluate the stress response of wild-type, ΔfliC, and ΔcsgG mutant E. coli exposed to charged surfaces. Substrate surface characterization and biological assays were used to correlate the physiological response to substrate surface charge. The physiological response was evaluated by measuring the intracellular levels of reactive oxygen species (ROS) and Ca²⁺ cations using fluorescent probes. We evaluated the response 1, 2, and 3 h after a short exposure to the surfaces to determine generational effects of the initial exposure on the physiology of the bacteria. In general, the ROS levels 1 h after exposure were not different. However, there were differences in Ca²⁺ levels in E. coli 1 h after the initial exposure to charged GaN surfaces, primarily in the wild-type E. coli. The differences in Ca²⁺ levels depended on the substrate surface chemistry and genetic mutation that suggests the involvement of multiple factors for modulating the interactions of bacteria at interfaces.

中文翻译：

使用表面电荷、表面化学和基因突变调节大肠杆菌在 GaN 界面处的应力响应

无机材料的表面性质可用于调节微生物在界面处的反应。我们使用化学处理的氮化镓基板的持久光电导特性来评估野生型、ΔfliC 和 ΔcsgG 突变体大肠杆菌暴露于带电表面的应力响应。基材表面表征和生物测定用于关联与基材表面电荷的生理反应。通过测量细胞内活性氧 (ROS) 和 Ca ^{2+的水平来评估生理反应}使用荧光探针检测阳离子。我们评估了短暂暴露于表面后 1、2 和 3 小时的反应，以确定初始暴露对细菌生理的世代影响。一般来说，暴露后 1 小时的 ROS 水平没有差异。然而，在最初暴露于带电 GaN 表面 1 小时后，大肠杆菌中的 Ca ²⁺水平存在差异，主要是在野生型大肠杆菌中。Ca ²⁺水平的差异取决于底物表面化学和基因突变，这表明多种因素参与调节界面处细菌的相互作用。

更新日期：2020-10-21

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