当前位置: X-MOL 学术Prep. Biochem. Biotechnol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Development of a fluorometric measurement system used in biological samples upon the determination of iron (II) metal ion
Preparative Biochemistry & Biotechnology ( IF 2.0 ) Pub Date : 2020-09-16 , DOI: 10.1080/10826068.2020.1818257
Yavuz Selim Toksöz 1 , İbrahim Ethem Özyiğit 1 , Çiğdem Bilen 1 , Nergis Arsu 1 , Emine Karakuş 1
Affiliation  

Abstract

2-thioxanthone thioacetic acid (TXSCH2COOH, T), which has a fluorometric character, was used for new fluorometric system upon Fe(II) analysis in biological samples as the main target. T-BSA binary complex was firstly consisted with non-covalent interactions between T and BSA at the equilibrium concentration as 1.77 × 10-4.M. T-BSA binary complex emission was increased at the ratio of 24.40% due to stabilization property of BSA (pH:7), compared with T emission intensity. Fluorescence emission spectroscopy was used for the all measurements because of an economic, a sensitive and a practical method compared with other spectroscopic analysis. T-BSA-Fe(II) triple complex was also obtained by adding Fe(II) ion to T-BSA binary complex solution. Its characterization was performed to be investigated with optimum excitation wavelength, buffer concentration, pH and temperature as 297 nm, 10-3 M Tris HCl (10-2M NaCI), pH:7.2 at 25 °C, respectively. The results of Fe(II) analysis in serum showed a certain response in fluorometric T-BSA-Fe(II) triple complex measurement system as 50.42 ± 5.8 µg/dL. The analyses of our fluorometric triple complex system were compared with the reference electrochemiluminescence method and similar results were obtained. Fluorometric measurements of T-BSA-Fe(II) triple complex, its characterization and Fe(II) analysis in this system have not been investigated in literature gives originality to our study.



中文翻译:

确定铁(II)金属离子后,用于生物样品的荧光测量系统的开发

摘要

具有荧光特征的2-噻吨酮硫代乙酸(TXSCH 2 COOH,T)被用于以生物样品中的Fe(II)分析为主要目标的新型荧光系统。T-BSA二元复合物首先由T和BSA之间的非共价相互作用组成,平衡浓度为1.77×10 -4。与T发射强度相比,由于BSA的稳定特性(pH:7),M。T-BSA二元复合物发射以24.40%的比率增加。与其他光谱分析相比,由于经济,灵敏和实用的方法,荧光发射光谱法可用于所有测量。通过将Fe(II)离子添加到T-BSA二元络合物溶液中,也获得了T-BSA-Fe(II)三元络合物。进行了表征,以最佳激发波长,缓冲液浓度,pH和温度为297 nm,10 -3 M Tris HCl(10 -2M NaCl),pH分别在25°C下为7.2。血清中的Fe(II)分析结果表明,在荧光T-BSA-Fe(II)三重络合物测量系统中,其响应为50.42±5.8 µg / dL。我们的荧光三重配合物系统的分析与参考电致发光方法进行了比较,并获得了相似的结果。T-BSA-Fe(II)三元络合物的荧光测定,该体系的表征和Fe(II)分析尚未在文献中进行研究,这为我们的研究提供了独创性。

更新日期:2020-09-16
down
wechat
bug