Abstract

2-thioxanthone thioacetic acid (TXSCH₂COOH, T), which has a fluorometric character, was used for new fluorometric system upon Fe(II) analysis in biological samples as the main target. T-BSA binary complex was firstly consisted with non-covalent interactions between T and BSA at the equilibrium concentration as 1.77 × 10^-4.M. T-BSA binary complex emission was increased at the ratio of 24.40% due to stabilization property of BSA (pH:7), compared with T emission intensity. Fluorescence emission spectroscopy was used for the all measurements because of an economic, a sensitive and a practical method compared with other spectroscopic analysis. T-BSA-Fe(II) triple complex was also obtained by adding Fe(II) ion to T-BSA binary complex solution. Its characterization was performed to be investigated with optimum excitation wavelength, buffer concentration, pH and temperature as 297 nm, 10^-3 M Tris HCl (10^-2M NaCI), pH:7.2 at 25 °C, respectively. The results of Fe(II) analysis in serum showed a certain response in fluorometric T-BSA-Fe(II) triple complex measurement system as 50.42 ± 5.8 µg/dL. The analyses of our fluorometric triple complex system were compared with the reference electrochemiluminescence method and similar results were obtained. Fluorometric measurements of T-BSA-Fe(II) triple complex, its characterization and Fe(II) analysis in this system have not been investigated in literature gives originality to our study.

摘要

具有荧光特征的2-噻吨酮硫代乙酸（TXSCH ₂ COOH，T）被用于以生物样品中的Fe（II）分析为主要目标的新型荧光系统。T-BSA二元复合物首先由T和BSA之间的非共价相互作用组成，平衡浓度为1.77×10 ^-4。与T发射强度相比，由于BSA的稳定特性（pH：7），M。T-BSA二元复合物发射以24.40％的比率增加。与其他光谱分析相比，由于经济，灵敏和实用的方法，荧光发射光谱法可用于所有测量。通过将Fe（II）离子添加到T-BSA二元络合物溶液中，也获得了T-BSA-Fe（II）三元络合物。进行了表征，以最佳激发波长，缓冲液浓度，pH和温度为297 nm，10 ^-3 M Tris HCl（10 ^-2M NaCl），pH分别在25°C下为7.2。血清中的Fe（II）分析结果表明，在荧光T-BSA-Fe（II）三重络合物测量系统中，其响应为50.42±5.8 µg / dL。我们的荧光三重配合物系统的分析与参考电致发光方法进行了比较，并获得了相似的结果。T-BSA-Fe（II）三元络合物的荧光测定，该体系的表征和Fe（II）分析尚未在文献中进行研究，这为我们的研究提供了独创性。