Abstract A universal procedure of modeling chromatography separation, based on the plate model, has been developed. On each plate, the equilibrium between the analyte in the mobile and stationary phases is established. This equilibrium may be described by the concentration-dependent partition coefficient. The establishment of the equilibrium on each plate is followed by the displacement of the analyte in the mobile phase by one plate, establishment of new equilibrium, etc. The result is a series of elution curves with positions of maxima dependent on injected mass of the polymer. The procedure has been tested on modeling concentration effects on the basis of dependences of the partition coefficient on local concentrations on each plate. By comparison with the experiment, the slope of the concentration dependence of the partition coefficient of polystyrene in benzene was estimated. It is in qualitative agreement with the literature data obtained by a computer simulation of chromatography separation of polymers in good solvents. Graphical Abstract

中文翻译：

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板模型应用于尺寸排阻色谱中的浓度效应

摘要 开发了一种基于板模型的色谱分离建模通用程序。在每个板上，流动相和固定相中的分析物之间建立了平衡。这种平衡可以用浓度相关的分配系数来描述。在每个平板上建立平衡之后，流动相中的分析物被一个平板置换，建立新的平衡等。 结果是一系列洗脱曲线的最大值位置取决于聚合物的注入质量. 该程序已根据分配系数对每个板上局部浓度的依赖性对模拟浓度影响进行了测试。与实验相比，估计了聚苯乙烯在苯中的分配系数的浓度依赖性斜率。它与通过计算机模拟聚合物在良溶剂中的色谱分离获得的文献数据定性一致。图形概要