Tripartite transporters as mechano‐transmitters in periplasmic alternating‐access mechanisms,FEBS Letters

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Tripartite transporters as mechano‐transmitters in periplasmic alternating‐access mechanisms
FEBS Letters ( IF 3.0 ) Pub Date : 2020-10-04 , DOI: 10.1002/1873-3468.13929
Satoshi Murakami ₁ , Ui Okada ₁ , Hendrik W van Veen ₂

Affiliation

To remove xenobiotics from the periplasmic space, Gram‐negative bacteria utilise unique tripartite efflux systems in which a molecular engine in the plasma membrane connects to periplasmic and outer membrane subunits. Substrates bind to periplasmic sections of the engine or sometimes to the periplasmic subunits. Then, the tripartite machines undergo conformational changes that allow the movement of the substrates down the substrate translocation pathway to the outside of the cell. The transmembrane (TM) domains of the tripartite resistance‐nodulation‐drug‐resistance (RND) transporters drive these conformational changes by converting proton motive force into mechanical motion. Similarly, the TM domains of tripartite ATP‐binding cassette (ABC) transporters transmit mechanical movement associated with nucleotide binding and hydrolysis at the nucleotide‐binding domains to the relevant subunits in the periplasm. In this way, metabolic energy is coupled to periplasmic alternating‐access mechanisms to achieve substrate transport across the outer membrane.

中文翻译：

三方转运蛋白作为周质交替通路机制中的机械传递体

为了从周质空间去除外源性物质，革兰氏阴性细菌利用独特的三方外排系统，其中质膜中的分子引擎连接到周质和外膜亚基。底物与发动机的周质部分结合，有时与周质亚基结合。然后，三方机器经历构象变化，使底物沿着底物易位途径向下移动到细胞外。三方抗性-结瘤-抗药性 (RND) 转运蛋白的跨膜 (TM) 域通过将质子动力转化为机械运动来驱动这些构象变化。相似地，三方 ATP 结合盒 (ABC) 转运蛋白的 TM 结构域将与核苷酸结合和水解相关的机械运动传递到周质中的相关亚基。通过这种方式，代谢能量与周质交替访问机制耦合，以实现跨外膜的底物运输。

更新日期：2020-10-04

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