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Cordyceps cateniannulata, a novel entomopathogenic fungus to control Stenoma impressella Busck (Lepidoptera: Elachistidae) in Colombia
Journal of Applied Entomology ( IF 1.7 ) Pub Date : 2020-09-16 , DOI: 10.1111/jen.12818
Luis Guillermo Montes‐Bazurto 1 , Alex Enrique Bustillo‐Pardey 1 , Héctor Camilo Medina‐Cárdenas 1
Affiliation  

Stenoma impressella is one of the most important defoliator pests in oil palm plantations in Colombia. To identify an alternative method for its control was characterized biologically and molecularly two strains of Cordyceps cateniannulata (CPIsp1201 and IPIsp1201) and three strains of Beauveria bassiana (CPBb0502; CPBb0411; CPBb0404) against S. impressella larvae. Virulence was evaluated under laboratory conditions. In an oil palm leaflet, individual larvae obtained from the insect colony were inoculated with 5 μl of a conidial suspension containing 1 × 107 conidia/ml. The five strains were pathogenic against S. impressella larvae. CPIsp1201 and IPIsp1201 strains resulted in the highest mortality and were subsequently evaluated in two bioassays using a dose of 1 × 1013 conidia/ha. In the first bioassay, performed under shaded conditions, leaves of oil palms were infested with 75 larvae from the breeding/treatment. The second bioassay was performed in the field using natural populations. No differences were found between strains in both bioassays and the different dosages (5 × 1012, 1 × 1013, and 1.5 × 1013 conidia/ha). Finally, the two strains were evaluated under oil palm plantation conditions at a dose of 1 × 1013 conidia/ha in 126 naturally infested palms. Larval mortality caused by the strains IPIsp1201 and CPIsp1201 (79.5% and 70.5%, respectively) was higher than the natural mortality registered in the control (37.3%). Cordyceps cateniannulata used at 1 × 1013 conidia/ha was effective at controlling S. impressella.

中文翻译:

Cordyceps cateniannulata,一种控制哥伦比亚Stenoma impressella Busck(鳞翅目:Elachistidae)的新型昆虫病原真菌

Stenoma impressella 是哥伦比亚油棕种植园中最重要的落叶害虫之一。为了确定其控制的替代方法,从生物学和分子学上表征了两株连环虫草(CPIsp1201 和 IPIsp1201)和三株白僵菌(CPBb0502;CPBb0411;CPBb0404)针对 S. impressella 幼虫。在实验室条件下评估毒力。在油棕传单中,从昆虫群落获得的个体幼虫接种 5 μl 含有 1 × 107 分生孢子/ml 的分生孢子悬浮液。这五个菌株对 S. impressella 幼虫具有致病性。CPIsp1201 和 IPIsp1201 菌株的死亡率最高,随后在两种生物测定中使用 1 × 1013 分生孢子/公顷的剂量进行评估。在第一次生物测定中,在阴影条件下进行,油棕的叶子被来自育种/处理的 75 只幼虫侵染。第二次生物测定是在野外使用自然种群进行的。在生物测定和不同剂量(5 × 1012、1 × 1013 和 1.5 × 1013 分生孢子/公顷)中,菌株之间没有发现差异。最后,在油棕种植园条件下,以 1 × 1013 分生孢子/公顷的剂量在 126 株自然感染的棕榈树中对这两种菌株进行了评估。由 IPIsp1201 和 CPIsp1201 菌株引起的幼虫死亡率(分别为 79.5% 和 70.5%)高于对照中登记的自然死亡率(37.3%)。以 1 × 1013 分生孢子/公顷使用的连环虫草可有效控制 S. impressella。在两种生物测定和不同剂量(5 × 1012、1 × 1013 和 1.5 × 1013 分生孢子/公顷)中,菌株之间没有发现差异。最后,在油棕种植园条件下,以 1×1013 分生孢子/公顷的剂量在 126 株自然感染的棕榈树中对这两种菌株进行了评估。由 IPIsp1201 和 CPIsp1201 菌株引起的幼虫死亡率(分别为 79.5% 和 70.5%)高于对照中登记的自然死亡率(37.3%)。以 1 × 1013 分生孢子/公顷使用的连环虫草可有效控制 S. impressella。在生物测定和不同剂量(5 × 1012、1 × 1013 和 1.5 × 1013 分生孢子/公顷)中,菌株之间没有发现差异。最后,在油棕种植园条件下,以 1×1013 分生孢子/公顷的剂量在 126 株自然感染的棕榈树中对这两种菌株进行了评估。由 IPIsp1201 和 CPIsp1201 菌株引起的幼虫死亡率(分别为 79.5% 和 70.5%)高于对照中登记的自然死亡率(37.3%)。以 1 × 1013 分生孢子/公顷使用的连环虫草可有效控制 S. impressella。由 IPIsp1201 和 CPIsp1201 菌株引起的幼虫死亡率(分别为 79.5% 和 70.5%)高于对照中登记的自然死亡率(37.3%)。以 1 × 1013 分生孢子/公顷使用的连环虫草可有效控制 S. impressella。由 IPIsp1201 和 CPIsp1201 菌株引起的幼虫死亡率(分别为 79.5% 和 70.5%)高于对照中登记的自然死亡率(37.3%)。以 1 × 1013 分生孢子/公顷使用的连环虫草可有效控制 S. impressella。
更新日期:2020-09-16
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