RNA2-encoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post-transcriptional gene silencing.,Molecular Plant Pathology

当前位置： X-MOL 学术 › Mol. Plant Pathol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

RNA2-encoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post-transcriptional gene silencing.
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2020-09-16 , DOI: 10.1111/mpp.12979
Caterina Carpino _{1,

2} , Inmaculada Ferriol Safont ₁ , Laura Elvira-González _{1,

3} , Vicente Medina ₄ , Luis Rubio ₁ , Ezio Peri ₂ , Salvatore Davino ₂ , Luis Galipienso Torregrosa ₁

Affiliation

Broad bean wilt virus 1 (BBWV‐1, genus Fabavirus, family Secoviridae) is a bipartite, single‐stranded positive‐sense RNA virus infecting many horticultural and ornamental crops worldwide. RNA1 encodes proteins involved in viral replication whereas RNA2 encodes two coat proteins (the large and small coat proteins) and two putative movement proteins (MPs) of different sizes with overlapping C‐terminal regions. In this work, we determined the role played by the small putative BBWV‐1 MP (VP37) on virus pathogenicity, host specificity, and suppression of post‐transcriptional gene silencing (PTGS). We engineered a BBWV‐1 35S‐driven full‐length cDNA infectious clone corresponding to BBWV‐1 RNA1 and RNA2 (pBBWV1‐Wt) and generated a mutant knocking out VP37 (pBBWV1‐G492C). Agroinfiltration assays showed that pBBWV1‐Wt, as the original BBWV‐1 isolate, infected broad bean, tomato, pepper, and Nicotiana benthamiana, whereas pBBWV1‐G492C did not infect pepper and tomato systemically. Also, pBBWV1‐G492C induced milder symptoms in broad bean and N. benthamiana than pBBWV1‐Wt. Differential retrotranscription and amplification of the (+) and (−) strands showed that pBBWV1‐G492C replicated in the agroinfiltrated leaves of pepper but not in tomato. All this suggests that VP37 is a determinant of pathogenicity and host specificity. Transient expression of VP37 through a potato virus X (PVX) vector enhanced PVX symptoms and induced systemic necrosis associated with programmed cell death in N. benthamiana plants. Finally, VP37 was identified as a viral suppressor of RNA silencing by transient expression in N. benthamiana 16c plants and movement complementation of a viral construct based on turnip crinkle virus (pTCV‐GFP).

中文翻译：

蚕豆枯萎病毒 1 号 RNA2 编码的 VP37 蛋白是致病性、宿主易感性的决定因素，也是转录后基因沉默的抑制因子。

蚕豆枯萎病毒 1 （BBWV-1，蚕豆病毒属， Secoviridae科）是一种二分体、单链正链 RNA 病毒，感染全世界许多园艺和观赏作物。 RNA1 编码参与病毒复制的蛋白质，而 RNA2 编码两种外壳蛋白（大外壳蛋白和小外壳蛋白）和两个具有重叠 C 末端区域的不同大小的推定运动蛋白 (MP)。在这项工作中，我们确定了小假定的 BBWV-1 MP (VP37) 对病毒致病性、宿主特异性和转录后基因沉默 (PTGS) 抑制的作用。我们设计了对应于 BBWV-1 RNA1 和 RNA2 (pBBWV1-Wt) 的 BBWV-1 35S 驱动的全长 cDNA 感染性克隆，并生成了敲除 VP37 的突变体 (pBBWV1-G492C)。农杆菌渗透测定表明，pBBWV1-Wt 作为原始 BBWV-1 分离株，感染蚕豆、番茄、辣椒和本塞姆氏烟草，而 pBBWV1-G492C 没有系统性感染辣椒和番茄。此外，pBBWV1-G492C 在蚕豆和本塞姆氏烟草中诱导的症状比 pBBWV1-Wt 更轻微。 (+) 和 (-) 链的差异逆转录和扩增表明，pBBWV1-G492C 在辣椒的农杆菌渗透叶子中复制，但不在番茄中复制。所有这些都表明 VP37 是致病性和宿主特异性的决定因素。通过马铃薯病毒 X (PVX) 载体瞬时表达 VP37 可增强本塞姆氏烟草植物中的 PVX 症状，并诱导与程序性细胞死亡相关的系统性坏死。最后，VP37 通过在N.Benthamiana 16c 植物和基于芜菁皱纹病毒 (pTCV-GFP) 的病毒构建体的运动互补。

更新日期：2020-10-13

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11