Depression‐like behavior is associated with lower Per2 mRNA expression in the lateral habenula of rats,Genes, Brain and Behavior

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Depression‐like behavior is associated with lower Per2 mRNA expression in the lateral habenula of rats
Genes, Brain and Behavior ( IF 2.4 ) Pub Date : 2020-09-16 , DOI: 10.1111/gbb.12702
Yang Li ₁ , Guangjian Li ₂ , Jicheng Li ₁ , Xuewei Cai ₁ , Yanfei Sun ₁ , Beilin Zhang ₁ , Hua Zhao _{1,

2}

Affiliation

Circadian rhythm dysfunction is primary symptom of depression and is closely related to depression onset. The role of the lateral habenula (LHb) of the thalamus in the pathogenesis of depression has been a research topic of great interest. The neuronal activity of this structure has circadian characteristics, which are related to the regulation of circadian rhythms. However, in depression model of rats, the role of clock genes in the LHb has not been assessed. To address this gap, we used a clomipramine (CLI) injection‐induced depression model in rats to assess the daily expression of rhythmic genes in the LHb and depression‐like behavior in rats at multiple time points. In determining the role of the Per2 gene in the development of depression‐like behavior in the LHb, we found that the expression of this clock gene differed in a circadian manner. Per2 expression was also significantly decreased in CLI‐treated rats in late afternoon (17:00) and in the middle of the night (1:00). Furthermore, silencing Per2 in the LHb of normal rats induced depression‐like behavior at night, suggesting that Per2 may play an important role in the pathogenesis of depression. Collectively, these results indicate that decreased Per2 expression in the LHb may be related to increased depression‐like behavior at night in depression model of rats.

中文翻译：

抑郁样行为与大鼠外侧缰核中 Per2 mRNA 表达降低有关

昼夜节律功能障碍是抑郁症的主要症状，与抑郁症发病密切相关。丘脑外侧缰核（LHb）在抑郁症发病机制中的作用一直是备受关注的研究课题。这种结构的神经元活动具有昼夜节律特征，与昼夜节律的调节有关。然而，在大鼠的抑郁模型中，尚未评估时钟基因在 LHb 中的作用。为了解决这一差距，我们在大鼠中使用氯米帕明 (CLI) 注射诱导的抑郁模型来评估 LHb 中节律基因的日常表达和大鼠在多个时间点的抑郁样行为。在确定Per2的作用基因在 LHb 中抑郁样行为的发展中，我们发现该时钟基因的表达以昼夜节律方式不同。在下午晚些时候（17:00）和半夜（1:00），CLI 治疗的大鼠中Per2的表达也显着降低。此外，沉默正常大鼠 LHb 中的Per2会在夜间诱发抑郁样行为，这表明Per2可能在抑郁症的发病机制中起重要作用。总的来说，这些结果表明LHb 中Per2表达降低可能与大鼠抑郁模型夜间抑郁样行为增加有关。

更新日期：2020-09-16

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