The effects of high-fat diet and metformin on urinary metabolites in diabetes and prediabetes rat models,Biotechnology and Applied Biochemistry

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The effects of high-fat diet and metformin on urinary metabolites in diabetes and prediabetes rat models
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-09-15 , DOI: 10.1002/bab.2021
Yan-Fen Lee _{1,

2} , Xuan-Yi Sim _{1,

2} , Ying-Hui Teh ₂ , Mohd Nazri Ismail ₃ , Peter Greimel ₄ , Vikneswaran Murugaiyah ₂ , Baharudin Ibrahim ₂ , Lay-Harn Gam _{1,

2}

Affiliation

High-fat diet (HFD) interferes with the dietary plan of patients with type 2 diabetes mellitus (T2DM). However, many diabetes patients consume food with higher fat content for a better taste bud experience. In this study, we examined the effect of HFD on rats at the early onset of diabetes and prediabetes by supplementing their feed with palm olein oil to provide a fat content representing 39% of total calorie intake. Urinary profile generated from liquid chromatography–mass spectrometry analysis was used to construct the orthogonal partial least squares discriminant analysis (OPLS-DA) score plots. The data provide insights into the physiological state of an organism. Healthy rats fed with normal chow (NC) and HFD cannot be distinguished by their urinary metabolite profiles, whereas diabetic and prediabetic rats showed a clear separation in OPLS-DA profile between the two diets, indicating a change in their physiological state. Metformin treatment altered the metabolomics profiles of diabetic rats and lowered their blood sugar levels. For prediabetic rats, metformin treatment on both NC- and HFD-fed rats not only reduced their blood sugar levels to normal but also altered the urinary metabolite profile to be more like healthy rats. The use of metformin is therefore beneficial at the prediabetes stage.

中文翻译：

高脂饮食和二甲双胍对糖尿病和糖尿病前期大鼠模型尿代谢物的影响

高脂肪饮食 (HFD) 会干扰 2 型糖尿病 (T2DM) 患者的饮食计划。然而，许多糖尿病患者食用脂肪含量较高的食物以获得更好的味蕾体验。在这项研究中，我们通过在饲料中补充棕榈油油以提供占总卡路里摄入量 39% 的脂肪含量，研究了 HFD 对糖尿病和糖尿病前期发病早期大鼠的影响。使用液相色谱-质谱分析生成的尿液分布图构建正交偏最小二乘判别分析 (OPLS-DA) 评分图。这些数据提供了对生物体生理状态的洞察。用正常食物 (NC) 和 HFD 喂养的健康大鼠无法通过其尿液代谢物谱来区分，而糖尿病和糖尿病前期大鼠在两种饮食之间的 OPLS-DA 谱中表现出明显的分离，表明它们的生理状态发生了变化。二甲双胍治疗改变了糖尿病大鼠的代谢组学特征并降低了它们的血糖水平。对于糖尿病前期大鼠，对 NC 和 HFD 喂养的大鼠进行二甲双胍治疗不仅将其血糖水平降低到正常水平，而且还改变了尿液代谢物谱，使其更像健康大鼠。因此，在糖尿病前期阶段使用二甲双胍是有益的。对 NC 和 HFD 喂养的大鼠进行二甲双胍治疗不仅将它们的血糖水平降低到正常水平，而且还改变了尿液代谢物谱，使其更像健康大鼠。因此，在糖尿病前期阶段使用二甲双胍是有益的。对 NC 和 HFD 喂养的大鼠进行二甲双胍治疗不仅将它们的血糖水平降低到正常水平，而且还改变了尿液代谢物谱，使其更像健康大鼠。因此，在糖尿病前期阶段使用二甲双胍是有益的。

更新日期：2020-09-15

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