In this work, a novel competitive electrochemical immunosensor was developed based on gold palladium bimetallic nanoparticles which were successfully synthesized and modified by amine (-NH₂) functionalized-nanoflower-like manganese oxide (NH₂-fMnO₂) for the rapid and sensitive detection of TBBPA in environmental waters. The MWCNTs modified GCE was employed to load TBBPA-antigens (Ag) through the effects of adsorption for the design of a highly sensitive immunosensor, which provided a large surface area for the specific recognition between antigen-antibody reactions. Meanwhile, AuPd-NH₂-fMnO_2, the signal amplifier was employed as a carrier tag to label secondary antibody (Ab₂), which showed excellent catalytic ability toward H₂O₂. The AuPd NPs boosted TBBPA oxidation, thereby generating improved signals. Hence, under optimal conditions, the competitive biosensor presented superior performances, as good sensitivity (LOD, 0.10 ng/mL; based on S/N = 3) and satisfactory accuracy (recoveries, 84-120%; CV, 2.35-6.24%), the proposed method was employed to analyze TBBPA in waters from various sources, indicating a great potential for the sensitive determination of trace TBBPA in aquatic environments.

在这项工作中，开发了一种新型的竞争性电化学免疫传感器，该传感器基于金钯双金属纳米粒子，该纳米粒子已成功通过胺（-NH ₂）功能化的纳米花状氧化锰（NH ₂ -fMnO ₂）合成和修饰，从而可以快速，灵敏地检测在环境水域中的TBBPA。MWCNTs修饰的GCE用于通过吸附作用加载TBBPA抗原（Ag），从而设计出高灵敏度的免疫传感器，从而为抗原抗体反应之间的特异性识别提供了较大的表面积。同时，信号放大器AuPd-NH ₂ -fMnO ₂被用作载体标签来标记二抗（Ab ₂），对H ₂ O ₂表现出优异的催化能力。AuPd NPs促进了TBBPA氧化，从而产生改善的信号。因此，在最佳条件下，竞争性生物传感器表现出优异的性能，具有良好的灵敏度（LOD，0.10 ng / mL；基于S / N = 3）和令人满意的准确度（回收率，84-120％； CV，2.35-6.24％）。 ，所提出的方法被用于分析各种来源的水中的六溴联苯，这在水生环境中灵敏测定痕量六溴联苯有很大的潜力。