Abstract The growth response of Phaseolus vulgaris to dual inoculation with arbuscular mycorrhizal (AM) fungi and rhizobia was studied in a low-fertility tropical soil in Madagascar. Two isolates of AM fungi identified as Acaulospora sp. and Glomus sp., respectively, along with a cocktail of ten Rhizobium spp. strains were used to conduct a greenhouse experiment in a fully randomized block design with two factors. The Phaseolus vulgaris seedlings received one of the following inoculation treatments: no inoculation, separate inoculation with each of the three microbial symbionts (the two AM fungal isolates and the rhizobia), and co-inoculation with each of the two AM fungal isolates and the mix of rhizobium strains. The results showed an additive effect of co-infection by AM fungi and rhizobia on plant growth and on the total N content of the plants, along with a synergistic effect on the total P content, the number of nodules and the mycorrhizal rate of the plants. Dual symbiosis with native strains contributes to the success of legumes, especially in harsh environments and low-fertility tropical soils.

中文翻译：

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用丛枝菌根真菌和根瘤菌双重接种种植在低肥力热带土壤中的菜豆的益处

摘要 在马达加斯加的低肥力热带土壤中研究了菜豆对丛枝菌根 (AM) 真菌和根瘤菌双重接种的生长反应。鉴定为 Acaulospora sp. 的两种 AM 真菌分离物。和 Glomus sp.，以及十种根瘤菌的鸡尾酒。菌株被用于在一个完全随机的区组设计中进行温室实验，其中包含两个因素。菜豆幼苗接受以下接种处理之一：不接种，分别接种三种微生物共生体（两种 AM 真菌分离物和根瘤菌），并与两种 AM 真菌分离物和混合物中的每一种共同接种根瘤菌菌株。结果表明，AM真菌和根瘤菌共感染对植物生长和植物总氮含量有累加作用，同时对植物总磷含量、根瘤数和菌根率有协同作用。 . 与本地菌株的双重共生有助于豆类的成功，尤其是在恶劣的环境和低肥力的热带土壤中。