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OXYGEN EXPOSURE IN EARLY LIFE ACTIVATES NLRP3 INFLAMMASOME IN MOUSE BRAIN.
Neuroscience Letters ( IF 2.5 ) Pub Date : 2020-09-16 , DOI: 10.1016/j.neulet.2020.135389
Serap Cilaker Micili 1 , Defne Engür 2 , Sermin Genc 3 , Ilkcan Ercan 3 , Sıla Soy 1 , Bora Baysal 4 , Abdullah Kumral 3
Affiliation  

Despite widely known detrimental effects on the developing brain, supplemental oxygen is still irreplaceable in the management of newborn infants with respiratory distress. Identifying downstream mechanisms underlying oxygen toxicity is a key step for development of new neuroprotective strategies. Main purpose of this study is to investigate whether NLRP3 inflammasome activation has a role in the pathogenesis of hyperoxia-induced preterm brain injury. C57BL6 pups were randomly divided into either a hyperoxia group (exposed to 90 % oxygen from birth until postnatal day 7) or control group (maintained in room air; 21 % O2). At postnatal day 7, all animals were sacrificed. Immunohistochemical examination revealed that hyperoxic exposure for seven days resulted in a global increase in NLRP3 and IL-1β immunopositive cells in neonatal mouse brain (p ≤ 0.001). There was a significant rise in Caspase-1 positive cell count in prefrontal and parietal area in the hyperoxia group when compared with controls (p ≤ 0.001). Western blot analysis of brain tissues showed elevated NLRP3, IL-1β and Caspase-1 protein levels in the hyperoxia group when compared with controls (p ≤ 0.001). To the best of our knowledge, this is the first study that investigates an association between hyperoxia and establishment of NLRP3 inflammasome in preterm brain.



中文翻译:

早期生命中的氧气暴露会引起小鼠脑中的NLRP3炎症。

尽管众所周知,这对发育中的大脑有害,但在处理呼吸窘迫的新生儿时,补充氧气仍然是不可替代的。确定潜在的氧气毒性下游机制是开发新的神经保护策略的关键步骤。这项研究的主要目的是调查NLRP3炎性小体活化是否在高氧引起的早产脑损伤的发病机理中起作用。将C57BL6幼犬随机分为高氧组(从出生到出生后第7天,暴露于90%的氧气)或对照组(保持在室内空气中; 21%的O 2))。在出生后第7天,将所有动物处死。免疫组织化学检查显示,高氧暴露7天导致新生小鼠脑中NLRP3和IL-1β免疫阳性细胞的总体增加(p≤0.001)。与对照组相比,高氧组的前额叶和顶叶区的Caspase-1阳性细胞计数显着增加(p≤0.001)。脑组织的蛋白质印迹分析显示,与对照组相比,高氧组的NLRP3,IL-1β和Caspase-1蛋白水平升高(p≤0.001)。据我们所知,这是第一项研究高氧与早产儿大脑中NLRP3炎性体的建立之间的关联的研究。

更新日期:2020-09-24
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