Infection associated with titanium based implants remains the most serious problem in implant surgery hence it is important to find optimal strategies to prevent infections. In the present study, we investigated the surface properties, antibacterial activity and biocompatibility of nanocomposite coatings based on an amorphous hydrocarbon (a-C:H) film containing copper nanoparticles (CuNPs) deposited on Ti discs via a gas aggregation cluster source. Three different Cu/a-C:H coatings with approximately the same amount of embedded CuNPs with and without barrier a-C:H layer were fabricated. The obtained results revealed that different structures of the produced coatings have significantly different release rates of Cu ions from the coatings into the aqueous media. This subsequently influences the antibacterial efficiency and osteoblast cell viability of the treated coatings. Coatings with the highest number of CuNPs resulted in excellent antibacterial activity exhibiting approximately 4 log reduction of E.coli and S.aureus after 24 h incubation. The cytotoxicity study revealed that after 7 day cell seeding, even the coating with the highest Cu at.% (4 at.%) showed a cell viability of ̴90%. Consequently, the coating, formed with a properly tailored number of CuNPs and a-C:H barrier thickness offer a strong antibacterial effect without any harm to osteoblast cells.

与钛基植入物相关的感染仍然是植入物外科手术中最严重的问题，因此找到预防感染的最佳策略非常重要。在本研究中，我们研究了基于无定形烃（aC：H）膜的纳米复合涂层的表面性能，抗菌活性和生物相容性，该膜包含通过气体聚集簇源沉积在Ti圆盘上的铜纳米颗粒（CuNPs）。制备了三种不同的Cu / aC：H涂层，具有大约相同数量的带有和不带有势垒aC：H层的嵌入式CuNP。获得的结果表明，所产生的涂层的不同结构具有显着不同的Cu离子从涂层向水性介质的释放速率。随后，这影响了处理过的涂层的抗菌效率和成骨细胞的生存能力。具有最高数量的CuNPs的涂层产生了优异的抗菌活性，表现出大约4 log的减少。孵育24小时后，大肠杆菌和金黄色葡萄球菌。细胞毒性研究表明，细胞接种7天后，即使具有最高Cu at。％（4 at。％）的涂层也显示出̴90％的细胞活力。因此，以适当定制数量的CuNP和aC：H阻挡层厚度形成的涂层具有很强的抗菌作用，而对成骨细胞没有任何伤害。