Abstract Hydrogen peroxide (H2O2), as a typical reactive oxygen species (ROS), has attracted increasing interest in biochemical field since its homeostasis has recognized implications with diverse health and diseases consequences. The detection of H2O2 is crucial to accurately understand its roles in the physiological and pathological process. Here, we develop a fluorescent probe BOD-H2O2 with its excitation and emission lying in the near infrared (NIR) region for highly sensitive and selective detecting H2O2. Upon treated with H2O2, BOD-H2O2 demonstrated a significant turn-on fluorescent signal and a high selectivity towards H2O2 over other cellular-abundant competitive ROS. The probe BOD-H2O2 can also be applied to visualize endogenous H2O2 with NIR cellular imaging, providing a potent tool for tracking the H2O2 in biological environments.

中文翻译：

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基于硼二吡咯甲烯的近红外荧光探针用于活细胞中过氧化氢的选择性成像和分析

摘要 过氧化氢 (H2O2) 作为一种典型的活性氧 (ROS)，由于其体内平衡已被认识到对多种健康和疾病后果的影响，因此在生化领域引起了越来越多的兴趣。H2O2 的检测对于准确了解其在生理和病理过程中的作用至关重要。在这里，我们开发了一种荧光探针 BOD-H2O2，其激发和发射位于近红外 (NIR) 区域，用于高灵敏度和选择性检测 H2O2。用 H2O2 处理后，BOD-H2O2 表现出显着的开启荧光信号和对 H2O2 的高选择性，而不是其他细胞丰富的竞争性 ROS。探针 BOD-H2O2 还可用于通过 NIR 细胞成像显示内源性 H2O2，为跟踪生物环境中的 H2O2 提供有效工具。