This study isolated CFI gene from Pelteobagrus fulvidraco and named it PfCFI. The cDNA of PfCFI is 2374 bp long, including a 52 bp 5′ untranslated sequence, a 222 bp 3′ untranslated sequence, and an open reading frame (ORF) of 2100 bp encoding polypeptide consisting of 699 amino acids. Phylogenetic analysis revealed that the PfCFI was closely related to CFI of Ictalurus punctatus. Real-time quantitative reverse transcription-PCR (qRT-PCR) analysis indicate that there is the PfCFI gene which expressed in all the rest of tested tissues in varied levels, and mainly distributed in liver and least in heart. The reseachers induce the expressions level of PfCFI gene in liver, spleen, head kidney and blood at different points in time after challenged with lipopolysaccharide (LPS), and polyriboinosinic polyribocytidylic acid (poly I:C), respectively. Together these results suggested that CFI gene plays an important role in resistance to pathogens in yellow catfish immunity.

本研究从黄颡鱼中分离出CFI基因，并将其命名为PfCFI。PfCFI的 cDNA长 2374 bp，包括一个 52 bp 的 5' 非翻译序列、一个 222 bp 的 3' 非翻译序列和一个 2100 bp 的开放阅读框 (ORF)，编码由 699 个氨基酸组成的多肽。系统发育分析表明，PfCFI与Ictalurus punctatus的CFI密切相关。实时定量逆转录-PCR(qRT-PCR)分析表明PfCFI基因在其余所有受试组织中均有不同程度的表达，主要分布在肝脏，最少见于心脏。研究者诱导PfCFI的表达水平分别用脂多糖 (LPS) 和多聚核糖胞苷酸 (poly I:C) 攻击后不同时间点的肝、脾、头肾和血液中的基因。这些结果共同表明CFI基因在黄鲶免疫病原菌抗性中起重要作用。