Plant Glycogen Synthase Kinase 3 (GSK3)/SHAGGY-like kinase (GSK) proteins play important roles in modulating growth, development, and stress responses in several plant species. However, little is known about the members of the potato GSK (StGSK) family. Here, nine StGSK genes were identified and phylogenetically grouped into four clades. Gene duplication analysis revealed that segmental duplication contributed to the expansion of the StGSK family. Gene structure and motif pattern analyses indicated that similar exon/intron and motif organizations were found in StGSKs from the same clade. Conserved motif and kinase activity analyses indicated that the StGSKs encode active protein kinases, and they were shown to be distributed throughout whole cells. Cis-acting regulatory element analysis revealed the presence of many growth-, hormone-, and stress-responsive elements within the promoter regions of the StGSKs, which is consistent with their expression in different organs, and their altered expression in response to hormone and stress treatments. Association network analysis indicated that various proteins, including two confirmed BES1 family transcription factors, potentially interact with StGSKs. Overexpression of StSK21 provides enhanced sensitivity to salt stress in Arabidopsis thaliana plants. Overall, these results reveal that StGSK proteins are active protein kinases with purported functions in regulating growth, development, and stress responses.

植物糖原合成酶激酶3（GSK3）/ SHAGGY样激酶（GSK）蛋白在调节几种植物物种的生长，发育和胁迫响应中起重要作用。但是，关于马铃薯GSK（StGSK）家族的成员知之甚少。在这里，确定了九个StGSK基因，并在系统发育上分为四个进化枝。基因重复分析表明，节段重复促进了StGSK家族的扩大。基因结构和基序模式分析表明，在同一进化枝的StGSK中发现了相似的外显子/内含子和基序组织。保守的基序和激酶活性分析表明StGSKs编码活性蛋白激酶，并且显示它们分布在整个细胞中。顺式作用调节元件分析显示，StGSK s启动子区域内存在许多生长，激素和应激反应元件，这与它们在不同器官中的表达一致，并且它们对激素和激素的响应也发生了改变。压力治疗。关联网络分析表明，各种蛋白质（包括两个已确认的BES1家族转录因子）可能与StGSKs相互作用。StSK21的过表达增强了拟南芥对盐胁迫的敏感性植物。总体而言，这些结果表明StGSK蛋白是活性蛋白激酶，据称具有调节生长，发育和应激反应的功能。