Organochlorine pesticides (OCPs) used in agricultural practices are of global concern due to their toxicological hazards on biomes of the impacted soil. Geochemistry and microbiome of OCPs-impacted (OW) soil was determined and compared with those of pristine (L1) soils. Microbiome of OW was based on sequencing total 16S rRNA genes of prokaryotes and Internal Transcribed Spacer (ITS2) regions between 5.8S and 28S rRNA genes of eukaryotes using Illumina MiSeq platform for bacterial and fungal communities, respectively. Geochemical properties of OW were assessed for ecological risks of OCPs on biota via risk quotient (RQ) and maximum cumulative ratio (MCR). It was established OW was polluted with 15 OCPs, along with consequential nitrate and phosphorous deficiencies. Ten of the 15 OCPs exerted severe ecological risk (RQ > 1: 4–992), of which endosulfan contributed 76% of the ecotoxicity (MCR = 1.3) on OW. The key players in OW were observed to be Enterobacteriaceae and Mortierellaceae represented by Escherichia and Mortierella taxa, respectively. Low abundance of Nitrospirae species and extinction of Glomeromycota in OW connoted serious toxicological consequences of the OCPs. Taxon XOR (Taxon Exclusive Or) analysis revealed 38,212 and 63,474 counts of bacterial and fungal species, respectively, were exclusively found in the impacted OW and possibly contributed to natural attenuation of the OCPs in the impacted agricultural soil. Conversely, 61,005 (bacteria) and 33,397 (fungi) species counts that were missing in OCPs-impacted OW, but present in pristine L1, opined the species as bio-indicators of OCPs ecotoxicity in agricultural soils. While the species tagged as bio-indicators would be valuable in monitoring OCPs pollution, those suggested to be players in self-recovery process will be invaluable to designing bioremediation strategies for OCPs-impacted agricultural soil.

农业实践中使用的有机氯农药（OCPs）由于其对受影响土壤生物群系的毒理危害而备受全球关注。确定了受OCPs影响的土壤（OW）的地球化学和微生物组，并与原始（L1）土壤的地球化学和微生物组进行了比较。OW的微生物组基于真核生物的总16S rRNA基因和真核生物的5.8S和28S rRNA基因之间的内部转录间隔区（ITS2）区域，分别使用Illumina MiSeq平台进行细菌和真菌群落测序。通过风险商（RQ）和最大累积比率（MCR）评估了OW的地球化学性质对生物群中OCP的生态风险。它被确定为OW被15种OCPs污染，以及随之而来的硝酸盐和磷缺乏症。15个OCP中有10个具有严重的生态风险（RQ> 1：4–992），其中硫丹对OW产生76％的生态毒性（MCR = 1.3）。据观察，OW中的主要参与者是肠杆菌科和Mortierellaceae，分别为大肠杆菌和被孢霉分别分类。OW中硝化螺旋菌物种的低丰度和glomeromycota的灭绝意味着OCP的严重毒理学后果。Taxon XOR（Taxon Exclusive Or）分析显示，分别在受影响的OW中仅发现了38,212和63,474的细菌和真菌物种计数，并且可能导致了受影响农业土壤中OCP的自然衰减。相反，在受OCP影响的OW中缺少61,005（细菌）和33,397（真菌）种计数，但存在于原始L1中，认为该种是OCP在农业土壤中生态毒性的生物指标。虽然被标记为生物指示剂的物种在监测OCP污染方面将很有价值，