The metal tolerance mechanism of plants is of great importance to explore the plant-based clean-up of environmental substrata contaminated by heavy metals. Indoor experiment of tobacco (Nicotiana tabacum L.) seedlings growing hydroponically in nutrient solution containing 0, 0.1, 0.5, 2.0, and 4.0 mg L⁻¹ V was conducted. The results indicated that plant overall growth performance was significantly affected at ≥ 2.0 mg L⁻¹ V. Oxidative stress degree as indicated by foliar O₂⁻· and H₂O₂ content intensified markedly at ≥ 0.5 mg L⁻¹ V treatments. In response, the plant activated its enzyme and non-enzyme protecting mechanism to cope with oxidative stress inflicted by vanadium. The activities of antioxidant enzymes, including SOD, POD, CAT, APX, and the concentration of non-enzyme antioxidants, e.g., AsA and GSH were all conspicuously (p < 0.5 or p < 0.1) enhanced at ≥ 0.5 mg L⁻¹ V treatments. Vanadium accumulated in leaves, stems, and roots increased with increasing vanadium level. The majority of the absorbed vanadium retained in plant root, and minor portions were transferred to aerial parts. Vanadium concentration in plant tissues ordered as root ˃ stem ˃ leaf. Translocation factors (TF) in vanadium-treated tobaccos (TF « 1) were significantly lower than that of control (TF ˃ 1). In conclusion, although vanadium at ≥ 2.0 mg L⁻¹ inhibited plant growth, tobacco exhibited a relatively good vanadium tolerance through self-adaptive regulation and has the potential as a phytostabilizer in decontaminating the environment contaminated by vanadium.

植物的金属耐受机制对于探索基于植物的重金属污染环境基质净化具有重要意义。对烟草( Nicotiana tabacum L.)幼苗在含有0、0.1、0.5、2.0和4.0 mg L ^-1 V的营养液中水培进行室内实验。结果表明，≥2.0 mg L ^-1 V处理时，植物整体生长性能受到显着影响。≥0.5 mg L ^-1 V处理时，以叶面O ₂ ⁻ ·和H ₂ O ₂含量表示的氧化应激程度显着增强。作为回应，植物激活其酶和非酶保护机制来应对钒造成的氧化应激。抗氧化酶（包括SOD、POD、CAT、APX）的活性以及非酶抗氧化剂（例如AsA和GSH）的浓度在≥0.5 mg L ^-1 V时均显着增强（ p < 0.5或p < 0.1）治疗。随着钒含量的增加，叶、茎和根中钒的积累也增加。吸收的钒大部分保留在植物根部，小部分转移到地上部分。植物组织中钒的浓度顺序为根˃茎˃叶。经钒处理的烟草中的易位因子 (TF) (TF < 1) 显着低于对照 (TF ˃ 1)。综上所述，虽然钒浓度≥2.0 mg·L ^-1会抑制植物生长，但烟草通过自适应调节表现出较好的耐钒性，具有作为植物稳定剂净化钒污染环境的潜力。