6-Hydroxydopamine (6-OHDA) is a neurotoxin that inhibits the mitochondrial complex I causing mitochondrial impairment, aetiology of Parkinson’s. Naringenin is a flavanone predominantly present in citrus fruits. Due to its high antioxidant and anti-inflammatory potential, it has been widely studied against various disorders. In this study, the neuroprotective effect of naringenin was determined against 6-OHDA induced toxicity with Levodopa (l-DOPA) as the standard. Naringenin reduced 6-OHDA induced oxidative stress biomarker levels such as CAT, GSH, SOD, and ROS. Naringenin rescued 6-OHDA induced reduction of the mitochondrial membrane potential. Treatment with naringenin improved the locomotion of the 6-OHDA treated zebrafish larvae which showed stagnant swimming patterns. Naringenin was also found to downregulate the expression of some Parkinsonian genes such as casp9, lrrk2, and polg and upregulate pink1. These studies attribute to naringenin as a viable molecule to study further for its neuroprotective effects against 6-OHDA induced neurotoxicity and neurodegeneration.

6-羟基多巴胺（6-OHDA）是一种神经毒素，可抑制线粒体复合体I，从而引起线粒体损害（帕金森氏病的病因）。柚皮苷是一种黄烷酮，主要存在于柑橘类水果中。由于其高抗氧化剂和抗发炎的潜力，已针对各种疾病进行了广泛的研究。在这项研究中，对6-OHDA诱导的毒性测定用左旋多巴（柚皮素的神经保护作用升-DOPA）作为标准。柚皮素降低了6-OHDA诱导的氧化应激生物标记物水平，例如CAT，GSH，SOD和ROS。柚皮素拯救了6-OHDA诱导的线粒体膜电位降低。柚皮素的处理改善了6-OHDA处理的斑马鱼幼虫的运动能力，显示出停滞的游泳模式。还发现柚皮苷可以下调一些帕金森病基因（如casp9，lrrk2和polg）的表达，并上调pink1。这些研究归因于柚皮苷作为一种可行的分子，以进一步研究其对6-OHDA诱导的神经毒性和神经变性的神经保护作用。