We present an approach for the elucidation of C=C bond position and cis/trans isomers, which is achieved by the reaction of ambient water radical cations and double bonds, followed by the fragmentation of epoxide radical cations to generate diagnostic ions in tandem mass spectrometry. Hexenol double bond positional isomers and cis/trans isomers which exhibit different properties and biological functions are characterized as a proof of concept. The merits of the approach include the simplicity of experimental setup, rapid derivatization (within seconds), the obviation of organic solvents, as well as easy spectral interpretation.

中文翻译：

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质谱法区分 C=C 位置和顺/反异构体：由水自由基阳离子引发的策略

我们提出了一种阐明 C=C 键位置和顺式/反式异构体的方法，这是通过环境水自由基阳离子和双键的反应实现的，然后环氧化物自由基阳离子的碎裂以串联质谱法产生诊断离子. 表现出不同性质和生物学功能的己烯醇双键位置异构体和顺/反异构体被表征为概念证明。该方法的优点包括实验设置简单、快速衍生（在几秒钟内）、无需有机溶剂以及易于光谱解释。