In this study, we report the development and characterization of 15 new microsatellite markers for Stryphnodendron adstringens (Leguminosae) in order to support future analyses of genetic diversity in populations of this species. In screening with 48 individuals from three different populations of S. adstringens, we tested the amplification of 20 microsatellite loci, of which five are not useful for population genetic studies due to the lack of polymorphisms or amplification failures. For the final set of 15 loci, the number of alleles ranged from 2 to 15, with a total of 116 alleles. The expected heterozygosity ranged from 0.1219 to 0.8965, with an average of 0.6694 per locus. The combined probability of genetic identity (PI = 8.12 × 10⁻¹⁵) and paternity exclusion (Q = 0.99999) estimations showed that the loci may be useful to discriminate between individuals of S. adstringens. Initial cross-amplification tests were satisfactory in three species of the genus Stryphnodendron: S. rotundifolium, S. coriaceum and S. polyphyllum. This new set of markers will be a useful tool for population genetic studies, contributing to the knowledge about the evolutionary history of S. adstringens and, additionally, correlated species.

在这项研究中，我们报告了Stryphnodendron adstringens（豆科）的 15 个新微卫星标记的开发和表征，以支持未来对该物种种群遗传多样性的分析。在筛选来自三个不同S. adstringens种群的 48 个个体时，我们测试了 20 个微卫星位点的扩增，其中 5 个由于缺乏多态性或扩增失败而对种群遗传研究没有用。对于最后一组 15 个基因座，等位基因的数量从 2 到 15 个不等，总共有 116 个等位基因。预期杂合度在 0.1219 到 0.8965 之间，每个基因座平均为 0.6694。遗传同一性的组合概率 ( PI  = 8.12 × 10 ⁻¹⁵) 和亲子排除 ( Q  = 0.99999) 估计表明该位点可能有助于区分S. adstringens 的个体。最初的交叉扩增试验在Stryphnodendron属的三个物种中令人满意：S. rotundifolium、S. coriaceum和S. polyphyllum。这组新标记将成为种群遗传研究的有用工具，有助于了解S. adstringens以及相关物种的进化历史。