Adipose-derived stem cells (ADSCs) are a type of mesenchymal stem cells with the therapeutic effects that make them one of the best sources for cell therapy. In this study, we aimed to assess the ability of human ADSCs for constant expression of IL-11 and IL-13, simultaneously. In this study, the characterized hADSCs were transduced with a lentiviral vector (PCDH-513B) containing IL-11 and IL-13 genes, and the ability of long-term expression of the transgenes was evaluated by ELISA technique on days 15, 45 and 75 after transduction. Our results indicated a high rate of transduction (more than 90%) in the isolated hADSCs. Our data showed the highest rate of expression on days 75 after transduction which was 242.67 pg/ml for IL-11 and 303.6 pg/ml for IL-13 compared with 35.2 pg/ml and 35.6 pg/ml in untreated cells, respectively (p = 0.001). Besides, MTT assay showed transduction of hADSCs with lentiviral viruses containing IL-11 and IL-13 had no adverse effect on hADSCs proliferation (p-value = 0.89). Finally, we successfully constructed a hADSC population stably overexpressing IL-11 as the neurotrophic cytokine and IL-13 as the anti-inflammatory cytokine and this transduced cells can be used for further studies in EAE mice model.

脂肪源性干细胞（ADSC）是一种间充质干细胞，具有治疗作用，使其成为细胞治疗的最佳来源之一。在本研究中，我们旨在评估人类 ADSC 同时恒定表达 IL-11 和 IL-13 的能力。在本研究中，用含有IL-11和IL-13基因的慢病毒载体（PCDH-513B）转导特征化的hADSC，并在第15天、第45天和第15天通过ELISA技术评估转基因的长期表达能力。转导后75。我们的结果表明分离的 hADSC 具有很高的转导率（超过 90%）。我们的数据显示转导后第 75 天的表达率最高，IL-11 为 242.67 pg/ml，IL-13 为 303.6 pg/ml，而未经处理的细胞分别为 35.2 pg/ml 和 35.6 pg/ml（p = 0.001）。此外，MTT实验显示，用含有IL-11和IL-13的慢病毒转导hADSCs对hADSCs增殖没有不利影响（p值=0.89）。最后，我们成功构建了稳定过表达IL-11作为神经营养细胞因子和IL-13作为抗炎细胞因子的hADSC群体，该转导细胞可用于EAE小鼠模型的进一步研究。