We conducted whole-genome sequencing of four inbred mouse strains initially selected for high (H1, H2) or low (L1, L2) open-field activity (OFA), and then examined strain distribution patterns for all DNA variants that differed between their BALB/cJ and C57BL/6J parental strains. Next, we assessed genome-wide sharing (3,678,826 variants) both between and within the High and Low Activity strains. Results suggested that about 10% of these DNA variants may be associated with OFA, and clearly demonstrated its polygenic nature. Finally, we conducted bioinformatic analyses of functional genomics data from mouse, rat, and human to refine previously identified quantitative trait loci (QTL) for anxiety-related measures. This combination of sequence analysis and genomic-data integration facilitated refinement of previously intractable QTL findings, and identified possible genes for functional follow-up studies.

我们对最初为高 (H1, H2) 或低 (L1, L2) 开放场活性 (OFA) 选择的四种近交小鼠品系进行了全基因组测序，然后检查了所有 BALB 之间不同的 DNA 变体的品系分布模式/cJ 和 C57BL/6J 亲本菌株。接下来，我们评估了高活性和低活性菌株之间和内部的全基因组共享（3,678,826 个变体）。结果表明，这些 DNA 变体中约有 10% 可能与 OFA 相关，并清楚地证明了其多基因性质。最后，我们对来自小鼠、大鼠和人类的功能基因组数据进行了生物信息学分析，以完善先前确定的数量性状基因座 (QTL)，用于与焦虑相关的测量。这种序列分析和基因组数据整合的结合促进了以前难以处理的 QTL 发现的改进，