Lung disease is a leading cause of morbidity and mortality. A breach in the lung alveolar-epithelial barrier and impairment in lung function are hallmarks of acute and chronic pulmonary illness. This review is part two of our previous work. In part 1, we demonstrated that CdM is as effective as MSCs in modulating inflammation. Herein, we investigated the effects of mesenchymal stromal cell (MSC)-conditioned media (CdM) on (i) lung architecture/function in animal models mimicking human lung disease, and (ii) performed a head-to-head comparison of CdM to MSCs. Adhering to the animal Systematic Review Centre for Laboratory animal Experimentation protocol, we conducted a search of English articles in five medical databases. Two independent investigators collected information regarding lung: alveolarization, vasculogenesis, permeability, histologic injury, compliance, and measures of right ventricular hypertrophy and right pulmonary pressure. Meta-analysis was performed to generate random effect size using standardized mean difference with 95% confidence interval. A total of 29 studies met inclusion. Lung diseases included bronchopulmonary dysplasia, asthma, pulmonary hypertension, acute respiratory distress syndrome, chronic obstructive pulmonary disease, and pulmonary fibrosis. CdM improved all measures of lung structure and function. Moreover, no statistical difference was observed in any of the lung measures between MSCs and CdM. In this meta-analysis of animal models recapitulating human lung disease, CdM improved lung structure and function and had an effect size comparable to MSCs.

中文翻译：

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间充质基质细胞条件培养基对肺结构和功能测量的影响：临床前研究的系统回顾和荟萃分析。

肺部疾病是发病和死亡的主要原因。肺泡上皮屏障的破坏和肺功能的损害是急性和慢性肺部疾病的标志。这篇评论是我们之前工作的第二部分。在第 1 部分中，我们证明了 CdM 在调节炎症方面与 MSC 一样有效。在此，我们研究了间充质基质细胞 (MSC) 条件培养基 (CdM) 对 (i) 模拟人类肺部疾病的动物模型中肺结构/功能的影响，以及 (ii) 对 CdM 与间充质干细胞。遵循实验动物实验动物系统审查中心协议，我们在五个医学数据库中检索了英文文章。两名独立研究人员收集了有关肺的信息：肺泡化、血管生成、渗透性、组织学损伤、顺应性以及右心室肥厚和右肺动脉压力的测量。使用具有 95% 置信区间的标准化均差进行荟萃分析以生成随机效应大小。共有 29 项研究被纳入。肺部疾病包括支气管肺发育不良、哮喘、肺动脉高压、急性呼吸窘迫综合征、慢性阻塞性肺病和肺纤维化。CdM 改善了肺结构和功能的所有指标。此外，MSC 和 CdM 之间的任何肺部测量结果均未观察到统计学差异。在这项重现人类肺部疾病的动物模型荟萃分析中，CdM 改善了肺结构和功能，并且具有与 MSC 相当的效应大小。