Chitinase and chitin-oligosaccaride can be used in multiple field, so it is important to develop a high-yield chitinase producing strain. Here, a recombinant Pichia pastoris with 4 copies of ChiA gene from Bacillus licheniformis and co-expression of molecular chaperon HAC1 was constructed. The amount of recombinant ChiA in the supernatant of high-cell-density fermentation reaches a maximum of 12.7 mg/mL, which is 24-fold higher than that reported in the previous study. The recombinant ChiA can hydrolyze 30% collodidal chitin with 74% conversion ratio, and GlcNAc is the most abundant hydrolysis product, followed by N, N′-diacetylchitobiose. Combined with BsNagZ, the hydrolysate of ChiA can be further transformed into GlcNAc with 88% conversion ratio. Additionally, the hydrolysate of ChiA can obviously accelerate the germination growth of rice and wheat, increasing the seedling height and root length by at least 1.6 folds within 10 days.

中文翻译：

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多种策略可提高巴斯德毕赤酵母中地衣芽孢杆菌的几丁质酶a产量，以获得植物生长促进剂和GlcNAc。

几丁质酶和几丁质寡糖可用于多个领域，因此开发高产几丁质酶的菌株很重要。在此，构建了具有地衣芽孢杆菌ChiA基因的4个拷贝并与分子伴侣HAC1共表达的重组毕赤酵母。高细胞密度发酵上清液中重组ChiA的量最大为12.7 mg / mL，比以前的研究报告高24倍。重组ChiA可以水解30％的胶体甲壳质，转化率为74％，GlcNAc是最丰富的水解产物，其次是N，N'-二乙酰基壳二糖。结合BsNagZ，ChiA的水解产物可以进一步转化为GlcNAc，转化率为88％。另外，