WRKY transcription factors (TFs) have been suggested to play crucial roles in the response to biotic and abiotic stresses. This study is the first to report the alkaline salt regulation of the WRKY gene. In this study, we cloned a WRKY gene (SlWRKY28) from the Salix linearistipularis and then transferred to the Populus davidiana × P. bolleana for expression. Sequence analysis on the transcriptome of Salix linearistipular showed the significant up-regulation of WRKY gene expression in response to salt-alkali stress in seedlings. Our data showed that SlWRKY28 localized to the nucleus. Furthermore, the expression of the SlWRKY28 from female plants increased with saline-alkali stress according to the northern blot analysis results. The results of 3,3′-Diaminobenzidine (DAB) staining showed that hydrogen peroxide (H2O2) concentration was lower under stress, but ascorbate peroxidase (APX) enzyme activity was significantly higher in the overexpressed plants than that in non-transgenic (NT) plants. We found out the SlWRKY28 induced regulation of the enzyme gene in the reactive oxygen species (ROS) scavenging pathway is a potential mechanism for transgenic lines to improve their resistance to alkaline salt. This study shows theoretical and practical significance in determining SlWRKY28 transcription factors involved in the regulation of alkaline salt tolerance.

中文翻译：

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转录因子SlWRKY28的过表达提高了山杨×波林杨对碱性盐胁迫的耐受性。


WRKY 转录因子（TF）被认为在生物和非生物胁迫响应中发挥着至关重要的作用。本研究首次报道了WRKY基因的碱性盐调控。本研究从柳树中克隆了一个WRKY基因(SlWRKY28)，然后将其转移到山杨×波里亚纳中进行表达。对线纹柳转录组的序列分析表明，幼苗响应盐碱胁迫，WRKY 基因表达显着上调。我们的数据显示 SlWRKY28 定位于细胞核。此外，根据Northern印迹分析结果，雌性植物中SlWRKY28的表达随着盐碱胁迫而增加。 3,3'-二氨基联苯胺（DAB）染色结果显示，胁迫下过表达植物过氧化氢（H2O2）浓度较低，但抗坏血酸过氧化物酶（APX）酶活性显着高于非转基因（NT）植物。植物。我们发现SlWRKY28诱导的活性氧（ROS）清除途径中酶基因的调节是转基因株系提高其对碱性盐的抗性的潜在机制。本研究对于确定参与碱性盐耐受性调节的SlWRKY28转录因子具有理论和实际意义。