A dual functional collagen scaffold coordinates angiogenesis and inflammation for diabetic wound healing,Biomaterials Science

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A dual functional collagen scaffold coordinates angiogenesis and inflammation for diabetic wound healing
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-09-15 , DOI: 10.1039/d0bm00999g
Ge Long ₁ , Dingyang Liu , Xi He , Yeyu Shen , Yannan Zhao , Xianglin Hou , Bing Chen , Wen OuYang , Jianwu Dai , Xing Li

Affiliation

Chronic diabetic wounds, which are associated with persistent inflammation and impaired angiogenesis, occur frequently in diabetic patients. Some studies have shown that separate administration of vascular endothelial growth factor (VEGF) or stromal cell derived factor 1α (SDF-1α) exhibited a therapeutic effect in promoting angiogenesis in the wound healing process. In this study, a collagen membrane is prepared as a drug delivery scaffold to investigate whether combined administration of SDF-1α and VEGF has a synergistic therapeutic effect on diabetic wound healing. We specifically fused a collagen-binding domain (CBD) with SDF-1α and VEGF separately, and sustained release of the two recombinant proteins from the collagen scaffold is successfully observed. Meanwhile, when a CBD-VEGF and CBD-SDF-1α co-modified scaffold is implanted in a diabetic rat skin wound model, it not only shows a synergistic effect in facilitating angiogenesis but also reduces inflammation in the short-term. Moreover, long-term results reveal that the co-modified scaffold is also able to enhance rapid wound healing, promote blood vessel regeneration, and assist cell proliferation, re-epithelialization and extracellular matrix accumulation. Taken together, our study indicates that the CBD-VEGF and CBD-SDF-1α co-modified scaffold helps in quick recovery from diabetic wounds by coordinating angiogenesis and inflammation.

中文翻译：

双功能胶原支架可协调糖尿病伤口愈合的血管生成和炎症

与持续性炎症和血管生成受损相关的慢性糖尿病伤口在糖尿病患者中经常发生。一些研究表明，分别给予血管内皮生长因子 (VEGF) 或基质细胞衍生因子 1α (SDF-1α) 在促进伤口愈合过程中的血管生成方面表现出治疗作用。在本研究中，制备胶原膜作为药物传递支架，以研究 SDF-1α 和 VEGF 联合给药对糖尿病伤口愈合是否具有协同治疗作用。我们将胶原结合结构域 (CBD) 与 SDF-1α 和 VEGF 分别特异性融合，并成功观察到两种重组蛋白从胶原支架中持续释放。同时，当将 CBD-VEGF 和 CBD-SDF-1α 共同修饰的支架植入糖尿病大鼠皮肤伤口模型时，它不仅在促进血管生成方面显示出协同作用，而且在短期内还可以减少炎症。此外，长期结果表明，共修饰支架还能够促进伤口快速愈合，促进血管再生，并有助于细胞增殖、再上皮化和细胞外基质积累。总之，我们的研究表明 CBD-VEGF 和 CBD-SDF-1α 共同修饰的支架通过协调血管生成和炎症有助于糖尿病伤口的快速恢复。长期结果表明，共修饰支架还能够促进伤口快速愈合，促进血管再生，并有助于细胞增殖、再上皮化和细胞外基质积累。总之，我们的研究表明 CBD-VEGF 和 CBD-SDF-1α 共同修饰的支架通过协调血管生成和炎症有助于糖尿病伤口的快速恢复。长期结果表明，共修饰支架还能够促进伤口快速愈合，促进血管再生，并有助于细胞增殖、再上皮化和细胞外基质积累。总之，我们的研究表明 CBD-VEGF 和 CBD-SDF-1α 共同修饰的支架通过协调血管生成和炎症有助于糖尿病伤口的快速恢复。

更新日期：2020-11-03

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