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Biopolymers-calcium phosphate antibacterial coating reduces the pathogenicity of internalized bacteria by mesenchymal stromal cells.
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-09-15 , DOI: 10.1039/d0bm00962h Marie Dubus 1 , Jennifer Varin-Simon , Pierre Prada , Loïc Scomazzon , Fany Reffuveille , Halima Alem , Fouzia Boulmedais , Cédric Mauprivez , Hassan Rammal , Halima Kerdjoudj
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-09-15 , DOI: 10.1039/d0bm00962h Marie Dubus 1 , Jennifer Varin-Simon , Pierre Prada , Loïc Scomazzon , Fany Reffuveille , Halima Alem , Fouzia Boulmedais , Cédric Mauprivez , Hassan Rammal , Halima Kerdjoudj
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A multifunctional material system that kills bacteria and drives bone healing is urgently sought to improve bone prosthesis. Herein, the osteoinductive coating made of calcium phosphate/chitosan/hyaluronic acid, named Hybrid, was proposed as an antibacterial substrate for stromal cell adhesion. This Hybrid coating possesses a contact-killing effect reducing by 90% the viability of Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Pseudomonas aeruginosa (P. aeruginosa) strains after 48 h of contact. In addition to the production of immunomodulatory mediators, Wharton's jelly (WJ-SCs), dental pulp (DPSCs) and bone marrow (BM-MSCs) derived stromal cells were able to release antibacterial and antibiofilm agents effective against S. aureus and P. aeruginosa strains, respectively. Studying the effect of the Hybrid coating on the internalization of S. aureus by the stromal cells, in acute-mimicking bone infection, highlighted an increase in the bacteria internalization by DPSCs and BM-MSCs when cultured on the Hybrid coating versus uncoated glass. Despite the internalization, Hybrid coating showed a beneficial effect by reducing the pathogenicity of the internalized bacteria. The formation of biofilm was reduced by at least 50% in comparison to internalized bacteria by stromal cells on uncoated glass. This work opens the route for the development of innovative antibacterial coatings by taking into account the internalization of bacteria by stromal cells.
中文翻译：
生物聚合物-磷酸钙抗菌涂层可降低间质基质细胞对内化细菌的致病性。
迫切需要一种能杀死细菌并促进骨骼愈合的多功能材料系统，以改善骨骼的修复效果。在此，提出了由磷酸钙/壳聚糖/透明质酸制成的骨诱导涂层，称为杂合体，作为用于基质细胞粘附的抗菌底物。这种混合涂层具有接触杀灭作用，可将革兰氏阳性金黄色葡萄球菌（S. aureus）和革兰氏阴性铜绿假单胞菌（P. aeruginosa）的活力降低90％）接触48小时后的菌株。除了产生免疫调节介质外，沃顿氏胶（WJ-SC），牙髓（DPSC）和骨髓（BM-MSC）衍生的基质细胞还可以释放出对金黄色葡萄球菌和铜绿假单胞菌有效的抗菌和抗生物膜剂。株。研究混合涂层的效果上的内在化的金黄色葡萄球菌由基质细胞，在急性模仿骨感染，关于混合涂层培养时强调了在由牙髓干细胞和BM-MSC的细菌内化的增加与未镀膜的玻璃。尽管有内在化作用，但杂化涂层通过降低内在化细菌的致病性显示出有益的作用。与未镀膜玻璃上的基质细胞内化细菌相比，生物膜的形成减少了至少50％。考虑到基质细胞对细菌的内在化，这项工作为开发创新的抗菌涂层开辟了道路。
更新日期：2020-10-13
中文翻译：
生物聚合物-磷酸钙抗菌涂层可降低间质基质细胞对内化细菌的致病性。
迫切需要一种能杀死细菌并促进骨骼愈合的多功能材料系统，以改善骨骼的修复效果。在此，提出了由磷酸钙/壳聚糖/透明质酸制成的骨诱导涂层，称为杂合体，作为用于基质细胞粘附的抗菌底物。这种混合涂层具有接触杀灭作用，可将革兰氏阳性金黄色葡萄球菌（S. aureus）和革兰氏阴性铜绿假单胞菌（P. aeruginosa）的活力降低90％）接触48小时后的菌株。除了产生免疫调节介质外，沃顿氏胶（WJ-SC），牙髓（DPSC）和骨髓（BM-MSC）衍生的基质细胞还可以释放出对金黄色葡萄球菌和铜绿假单胞菌有效的抗菌和抗生物膜剂。株。研究混合涂层的效果上的内在化的金黄色葡萄球菌由基质细胞，在急性模仿骨感染，关于混合涂层培养时强调了在由牙髓干细胞和BM-MSC的细菌内化的增加与未镀膜的玻璃。尽管有内在化作用，但杂化涂层通过降低内在化细菌的致病性显示出有益的作用。与未镀膜玻璃上的基质细胞内化细菌相比，生物膜的形成减少了至少50％。考虑到基质细胞对细菌的内在化，这项工作为开发创新的抗菌涂层开辟了道路。
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