Simvastatin (SIM) has been documented to induce the osteogenic differentiation of periodontal ligament stem cells (PDLSCs). To establish an efficient release system for periodontal regeneration, a polycaprolactone (PCL) membrane scaffold containing SIM was electrospun and evaluated. The obtained PCL-SIM membrane scaffold showed sustained release up to 28 days, without deleterious effect on proliferation of PDLSCs on the scaffolds. PDLSCs were seeded onto scaffolds and their osteogenic differentiation was evaluated. After 21 days, expressions of collagen type I, alkaline phosphatase and bone sialoprotein genes were significantly upregulated and mineralized matrix formation was increased on the PCL-SIM scaffolds compared with the PCL scaffolds. In a heterotopic periodontal regeneration model, a cell sheet-scaffold construct was assembled by placement of multilayers of PDLSC sheets on PCL or PCL-SIM scaffolds, and these were then placed between dentin and ceramic bovine bone for subcutaneous implantation in athymic mice. After 8 weeks, the PCL-SIM membrane showed formation of significantly more ectopic cementum-like mineral on the dentin surface. These findings demonstrated that the PCL-SIM membrane scaffold promotes cementum-like tissue formation by sustained drug release, suggesting the feasibility of its therapeutic use with PDLSC sheets to improve periodontal regeneration.

中文翻译：

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用于牙周再生的具有牙周膜干细胞片的辛伐他汀释放支架

辛伐他汀 (SIM) 已被证明可诱导牙周膜干细胞 (PDLSC) 的成骨分化。为了建立有效的牙周再生释放系统，对含有 SIM 的聚己内酯 (PCL) 膜支架进行了电纺和评估。获得的 PCL-SIM 膜支架显示持续释放长达 28 天，对支架上 PDLSCs 的增殖没有有害影响。PDLSCs 被接种到支架上并评估它们的成骨分化。21 天后，与 PCL 支架相比，PCL-SIM 支架上 I 型胶原蛋白、碱性磷酸酶和骨唾液蛋白基因的表达显着上调，矿化基质形成增加。在异位牙周再生模型中，通过在 PCL 或 PCL-SIM 支架上放置多层 PDLSC 片来组装细胞片 - 支架结构，然后将它们放置在牙本质和陶瓷牛骨之间，用于皮下植入无胸腺小鼠。8 周后，PCL-SIM 膜显示在牙本质表面形成明显更多的异位牙骨质样矿物质。这些发现表明 PCL-SIM 膜支架通过持续药物释放促进牙骨质样组织的形成，表明其与 PDLSC 片一起治疗使用以改善牙周再生的可行性。PCL-SIM 膜显示在牙本质表面形成明显更多的异位牙骨质样矿物质。这些发现表明 PCL-SIM 膜支架通过持续药物释放促进牙骨质样组织的形成，表明其与 PDLSC 片一起治疗使用以改善牙周再生的可行性。PCL-SIM 膜显示在牙本质表面形成明显更多的异位牙骨质样矿物质。这些发现表明 PCL-SIM 膜支架通过持续药物释放促进牙骨质样组织的形成，表明其与 PDLSC 片一起治疗使用以改善牙周再生的可行性。