Discriminative detection of GSH is achieved by employing a highly sensitive and selective fluorescent probe (KL-DN) that bears β-carboline alkaloid as a potential fluorophore and an azide group as the recognition unit. A rapid fluorescence off-on change is caused by special redox reaction; KL-DN has the capability of monitoring endogenous GSH in wheat germ tissues, indicating that this probe holds great potential for biological applications in plant tissues.

中文翻译：

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高效的基于β-咔啉生物碱的小麦胚芽组织内源性谷胱甘肽成像技术

GSH的判别检测是通过使用高度敏感的选择性荧光探针（KL-DN）来实现的，该探针带有β-咔啉生物碱作为潜在的荧光团，并使用叠氮基作为识别单元。特殊的氧化还原反应引起荧光的快速变化。KL-DN具有监测小麦胚芽组织中内源性GSH的能力，表明该探针在植物组织中的生物学应用方面具有巨大潜力。