The demand for vitamin D analysis in veterinary species is increasing with the growing knowledge of the extra-skeletal role vitamin D plays in health and disease. The circulating 25-hydroxyvitamin-D (25(OH)D) metabolite is used to assess vitamin D status, and the benefits of analysing other metabolites in the complex vitamin D pathway are being discovered in humans. Profiling of the vitamin D pathway by liquid chromatography tandem mass spectrometry (LC-MS/MS) facilitates simultaneous analysis of multiple metabolites in a single sample and over wide dynamic ranges, and this method is now considered the gold-standard for quantifying vitamin D metabolites. However, very few studies report using LC-MS/MS for the analysis of vitamin D metabolites in veterinary species. Given the complexity of the vitamin D pathway and the similarities in the roles of vitamin D in health and disease between humans and companion animals, there is a clear need to establish a comprehensive, reliable method for veterinary analysis that is comparable to that used in human clinical practice. In this review, we highlight the differences in vitamin D metabolism between veterinary species and the benefits of measuring vitamin D metabolites beyond 25(OH)D. Finally, we discuss the analytical challenges in profiling vitamin D in veterinary species with a focus on LC-MS/MS methods.

中文翻译：

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兽医物种中的维生素 D 代谢和分析。


随着人们对维生素 D 在健康和疾病中发挥的骨骼外作用的认识不断加深，对兽医物种维生素 D 分析的需求也在不断增加。循环 25-羟基维生素-D (25(OH)D) 代谢物用于评估维生素 D 状态，并且在人类中发现了分析复杂维生素 D 途径中其他代谢物的好处。通过液相色谱串联质谱 (LC-MS/MS) 分析维生素 D 途径有助于在宽动态范围内同时分析单个样品中的多种代谢物，该方法现在被认为是量化维生素 D 代谢物的黄金标准。然而，很少有研究报告使用 LC-MS/MS 分析兽医物种中的维生素 D 代谢物。鉴于维生素 D 途径的复杂性以及维生素 D 在人类和伴侣动物健康和疾病中的作用的相似性，显然需要建立一种全面、可靠的兽医分析方法，该方法与人类使用的分析方法相当。临床实践。在这篇综述中，我们强调了兽医物种之间维生素 D 代谢的差异以及测量 25(OH)D 以上维生素 D 代谢物的好处。最后，我们讨论了兽医物种中维生素 D 分析的分析挑战，重点关注 LC-MS/MS 方法。