Rhodamine-Appended Polymeric Probe: An Efficient Colorimetric and Fluorometric Sensing Platform for Hg2+ in Aqueous Medium and Living Cells,ACS Applied Polymer Materials

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Rhodamine-Appended Polymeric Probe: An Efficient Colorimetric and Fluorometric Sensing Platform for Hg2+ in Aqueous Medium and Living Cells
ACS Applied Polymer Materials ( IF 4.4 ) Pub Date : 2020-09-15 , DOI: 10.1021/acsapm.0c00878
Neha Choudhury ₁ , Bhuban Ruidas ₂ , Chitrangada Das Mukhopadhyay ₂ , Priyadarsi De ₁

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Nowadays, monitoring of trace amounts of Hg²⁺ ions in environmental and biological systems has become crucial owing to their detrimental effects on living organisms. To serve this purpose, a rhodamine-affixed water-soluble polymeric probe, CP2, was fabricated to detect Hg²⁺ ions in water and living cells. The sensing performance toward several competitive cations was assessed via UV–vis spectroscopy and fluorescence spectroscopy, which indicated high selectivity of the polymeric probe toward Hg²⁺ ions. CP2 showed both colorimetric (colorless to pink to violet) and fluorimetric “off–on” (nonemissive to red fluorescence) responses toward Hg²⁺ in water at pH 7.0 with a low limit of detection (10.7 nM) and a high binding constant value (7.14 × 10⁷ M^–1). The spectral changes were ascribed to Hg²⁺-induced spirolactam ring-opening of xanthene units present in the polymeric pendant. CP2 readily binds (less than a minute) with Hg²⁺ ions through a 1:1 irreversible complex formation. For practical applicability, filter-paper-based test strips were fabricated and utilized for naked-eye sensing of Hg²⁺ ions in water. Moreover, intracellular imaging applications demonstrated high efficiency of CP2 as a biocompatible fluorescent probe to sense Hg²⁺ ions in glioblastoma cells (U-87).

中文翻译：

罗丹明附加聚合物探针：高效比色和荧光传感平台，用于水介质和活细胞中的Hg ²⁺

如今，由于环境和生物系统中的痕量Hg ²⁺对生物体具有有害作用，因此对其进行监测已变得至关重要。为了达到这个目的，制造了若丹明固定的水溶性聚合物探针CP2，以检测水和活细胞中的Hg ²⁺离子。通过紫外可见光谱和荧光光谱评估了对几种竞争性阳离子的传感性能，这表明聚合物探针对Hg ²⁺离子具有很高的选择性。CP2对Hg ²⁺表现出比色（从无色到粉红色到紫色）和荧光“关-开”（不发射红色荧光）响应。在pH 7.0的水中，检测限低（10.7 nM），结合常数高（7.14×10 ⁷ M ^–1）。光谱变化归因于存在于聚合物侧链中的g吨单元的Hg ²⁺诱导的螺内酰胺开环。CP2可以通过1：1不可逆的络合物形成轻松地（不到一分钟）与Hg ²⁺离子结合。为了实际应用，制造了基于滤纸的测试条并将其用于肉眼检测水中的Hg ²⁺离子。此外，细胞内成像应用证明了CP2作为生物相容性荧光探针可检测Hg ^2+的高效性胶质母细胞瘤细胞中的离子（U-87）。

更新日期：2020-11-13

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